两种穗伸出差异显著的大麦最上节间全长转录组分析

doi:10.16036/j.issn.1000-2650.2021.04.006

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (4): 459-466.doi: 10.16036/j.issn.1000-2650.2021.04.006

两种穗伸出差异显著的大麦最上节间全长转录组分析

蒲茜^1,2, 邱雪冰^1,2, 张镌钰^1,2, 汤燕燕^1,2, 王金慧¹, 李涛¹, 杨钊^1,2, 张海莉¹, 梁俊俊¹, 唐亚伟³, 邓光兵^1,*, 龙海^1,*

1.中国科学院成都生物研究所,成都 610041;
2.中国科学院大学,北京 101408;
3.西藏自治区农牧科学院,西藏自治区拉萨 850000

收稿日期:2021-04-06 出版日期:2021-08-30 发布日期:2021-08-30
通讯作者: *龙海,副研究员,主要从事麦类作物高产、稳产形成的分子基础解析研究,E-mail：hailong@cib.ac.cn;邓光兵,副研究员,主要从事植物育种、植物细胞与分子生物学研究,E-mail：denggb@cib.ac.cn。
作者简介:蒲茜,硕士研究生。
基金资助:
中国科学院科技服务网络计划（STS计划,KFJ-STS-QYZD-2021-22-001）; 四川省科技支撑计划“四川省农作物分子育种平台项目”（2016NZ0103）; 西藏自治区重大科技专项（XZ2021NA01）; 四川省科技厅-应用基础研究项目（2019YZ0011）

Full-Length Transcriptome Sequencing Analysis of Uppermost Internode of Two Barley Accessions Significantly Differed in Spike Exsertion

PU Xi^1,2, QIU Xuebing^1,2, ZHANG Juanyu^1,2, TANG Yanyan^1,2, WANG Jinhui¹, LI Tao¹, YANG Zhao^1,2, ZHANG Haili¹, LIANG Junjun¹, TANG Yawei³, DENG Guangbing^1,*, LONG Hai^1,*

1. Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;
2. University of Chinese Academy of Sciences,Beijing 101408,China;
3. Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,Tibet,850000,China

Received:2021-04-06 Online:2021-08-30 Published:2021-08-30

摘要/Abstract

摘要： 【目的】对两份穗伸出差异显著的大麦（Hordeum vulgare L.）材料的最上节间（UI）进行全长转录组分析,为揭示大麦UI伸长调控机制奠定基础。【方法】以包穗大麦材料CDB0012和穗伸出正常大麦品种XL19为材料,利用Nanopore平台开展了UI全长转录组测序和生物信息分析。【结果】共获得46 246条非冗余全长转录本,鉴定了2 395个潜在新基因位点和44 805条新转录本,开展了功能注释、可变剪切、差异表达基因鉴定等分析。【结论】构建了一套高质量大麦UI全长转录组数据集。

关键词: 大麦, 最上节间, 全长转录组, 可变剪接, 长链非编码RNA

Abstract: 【Objective】 To conduct full-length transcriptome analysis of uppermost internode (UI) of two barley (Hordeum vulgare L.) accessions significantly differed by spike exsertion and build a foundation for further dissection of regulation mechanism underlying UI elongation. 【Method】 Two barley accessions,CDB00012 with sheathed spike and the Himalaya 19 (XL19) with normal spike exsertion, were used for full-length transcriptome sequencing on Nanopore platform and further bioinformatic analysis. 【Result】 A total of 46 246 unique full-length transcripts, 2 395 potential new gene loci and 44 805 new transcripts were identified, and in depth analyses, such as functional annotation, alternative splicing and differentially expressed genes, were also carried out. 【Conclusion】 Two UI high-quality full-length transcriptome datasets of barley were constructed.

Key words: barley, uppermost internode, full-length transcriptome, alternative splicing, long non-coding RNA (LncRNA)

中图分类号:

S512.3

蒲茜, 邱雪冰, 张镌钰, 汤燕燕, 王金慧, 李涛, 杨钊, 张海莉, 梁俊俊, 唐亚伟, 邓光兵, 龙海. 两种穗伸出差异显著的大麦最上节间全长转录组分析[J]. 四川农业大学学报, 2021, 39(4): 459-466.

PU Xi, QIU Xuebing, ZHANG Juanyu, TANG Yanyan, WANG Jinhui, LI Tao, YANG Zhao, ZHANG Haili, LIANG Junjun, TANG Yawei, DENG Guangbing, LONG Hai. Full-Length Transcriptome Sequencing Analysis of Uppermost Internode of Two Barley Accessions Significantly Differed in Spike Exsertion[J]. Journal of Sichuan Agricultural University, 2021, 39(4): 459-466.

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两种穗伸出差异显著的大麦最上节间全长转录组分析

Full-Length Transcriptome Sequencing Analysis of Uppermost Internode of Two Barley Accessions Significantly Differed in Spike Exsertion

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