四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (4): 423-426.doi: 10.16036/j.issn.1000-2650.2021.04.001

• 动物科学·动物医学 •    下一篇

姜黄素缓解毒死蜱对大口黑鲈氧化胁迫的分子机制

赵柳兰1#, 唐钢1#, 熊陈1#, 韩帅帅1, 杨春平2, 罗伟1, 李志琼1, 杨淞1,*   

  1. 1.四川农业大学动物科技学院,成都 611130;
    2.四川农业大学农学院,成都 611130
  • 收稿日期:2020-06-07 出版日期:2021-08-30 发布日期:2021-08-30
  • 通讯作者: *杨淞,教授,主要从事水产健康养殖研究,E-mail:ysys210@hotmail.com。
  • 作者简介:赵柳兰,副教授,主要从事水产养殖、鱼类生理及免疫适应性研究,E-mail:zhaoliulan2007@163.com。#为同等贡献。
  • 基金资助:
    国家重点研发“蓝色粮仓”渔农综合种养子项目(2019YFD0900305); 四川农业大学双支计划项目(2021993226)

The Molecular Mechanism of Curcumin Alleviating Oxidative Stress of Chlorpyrifos on Largemouth Bass (Micropterus salmoides)

ZHAO Liulan1#, TANG Gang1#, XIONG Chen1#, HAN Shuaishuai1, YANG Chunping2, LUO Wei1, LI Zhiqiong1, YANG Song1,*   

  1. 1. College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;
    2. College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2020-06-07 Online:2021-08-30 Published:2021-08-30

摘要: 【目的】鱼类常常受到非生物环境因子的胁迫,大田农药通过各种途径进入养殖池塘进而影响鱼类健康。解析日粮添加姜黄素缓解毒死蜱诱导大口黑鲈的氧化应激和免疫损伤的分子机制,对水产养殖可持续发展具有重要意义。【方法】利用生理、生化、蛋白杂交和GCMS等技术,结合姜黄素拌饵投喂处理,对慢性毒死蜱暴露下机体抗氧化、凋亡、炎症因子相关基因表达和毒死蜱富集规律进行了分析。【结果】慢性毒死蜱暴露抑制了大口黑鲈体重增长、导致组织损伤、扰乱抗氧化酶系统,并检测到肌肉中毒死蜱显著富集。通过上调促炎因子以及Caspase-3Caspase-8Caspase-9的表达诱导凋亡,促进炎症发生。日粮添加100 mg/kg姜黄素对大口黑鲈鱼进行投喂,能够增加鳃和肠中keap1的表达水平,减少Nrf2的表达水平。姜黄素能够显著上调肝中Nrf2以及TGF-β1IL-10Bcl-2的表达水平,起到抗凋亡和抗炎的作用。相关研究成果以“Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides)”为题于2021年6月在线发表于国际期刊Environmental Pollution (DOI: 10.1016/j.envpol.2021.117010)。【结论】研究成果表明慢性毒死蜱暴露虽诱导了氧化应激、凋亡和免疫紊乱,但姜黄素可降低鱼体农药富集水平,缓解由毒死蜱对大口黑鲈造成的氧化胁迫。

关键词: 大口黑鲈, 毒死蜱, 氧化胁迫, 姜黄素, 抗凋亡, 抗炎

Abstract: 【Objective】 Fish often suffer stress from abiotic environmental factors, obviously, pesticides can affect the health of fish farming through various ways in the pond system. In order to support the sustainable development of aquaculture, it is of great significance to analyze the effects of chlorpyrifos on oxidative stress and immune disorders of largemouth bass and confirm the molecular mechanism of dietary curcumin under chlorpyrifos toxicity. 【Method】 Combined with the feeding treatment of curcumin, the physiological, biochemical and GCMS techniques were adopt to analyze the expression of genes related to antioxidant, apoptosis, inflammatory factors, and also detect the chlorpyrifos enrichment in the muscle under chronic chlorpyrifos exposure. 【Result】 Chlorpyrifos toxicity led to body weight growth inhibition, histopathological lesions and antioxidase system disruption, and the accumulation concentration of chlorpyrifos in muscle were detected significantly. It induced apoptosis and promote inflammation by up-regulating the expression of pro-inflammatory factors and caspase-3, caspase-8 and caspase-9. The supplementation with 100 mg curcumin per kg diet significantly increased the expression level of Keap1 and decreased the expression level of Nrf2 in gill and intestine. Meanwhile, feeding on curcumin led to an improvement in the expression levels of TGF-β1, IL-10, Bcl-2 and Nrf2 in liver, which had anti-apoptotic and anti-inflammatory effects. The related results were published on Environmental Pollution in June, 2021 with the title “Chronic chlorpyrifos exposure induces oxidative stress, apoptosis and immune dysfunction in largemouth bass (Micropterus salmoides)” (doi.org/10.1016/j.envpol.2021.117010). 【Conclusion】 The results showed that chronic chlorpyrifos exposure induced oxidative stress, apoptosis and immune disorders, fortunately, curcumin can relieve the level of pesticide enrichment and alleviate oxidative stress caused by chlorpyrifos on largemouth bass.

Key words: Micropterus salmoides, chlorpyrifos, oxidative stress, curcumin, anti-apoptotic, anti-inflammatory

中图分类号: 

  • X171.5