幼龄和成年牦牛肌纤维类型、MyHC基因表达及其肌肉抗氧化能力的比较研究

doi:10.16036/j.issn.1000-2650.2021.02.017

摘要/Abstract

摘要： 【目的】研究幼龄和成年牦牛肌肉抗氧化能力和肌纤维组织学特性。【方法】以自然放牧条件下3月龄幼龄和3岁龄成年牦牛（♂）背最长肌作为试验材料,利用双抗体夹心法、ATP酶染色法和PCR技术进行测定。【结果】①成年牛背最长肌Ⅰ型、ⅡA型和ⅡB型肌纤维直径、横切面积均高于犊牛,但ⅡB型肌纤维直径差异不显著（P>0.05）,而犊牛背最长肌Ⅰ型、ⅡA型和ⅡB型肌纤维密度则显著高于成年牛（P<0.05）。②犊牛MyHCⅠ和MyHCⅡa基因的mRNA表达量显著成年牛（P<0.05）,而MyHCⅡb与MyHCⅡx基因mRNA表达量则呈现相反趋势（P<0.05）。③两组肌肉总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）和丙二醛（MDA）等指标均无显著差异（P>0.05）。【结论】3岁龄牦牛与3月龄牦牛相比,降低了氧化性肌纤维类型,影响其肉品质,同时抗氧化能力差异不显著。

关键词: 牦牛, 组织学特性, 肌球蛋白重链, ATP酶染色, 抗氧化指标

Abstract: 【Objective】 To study the antioxidant capacity in yak muscle and histology characteristics of muscle fiber. 【Method】 With natural grazing conditions in March and three yaks (came) back muscle as test material, using double antibody clip art, atpase staining method and real-time fluorescence quantitative polymerase chain reaction (polymerase chain reaction, PCR) technology were determined. 【Result】 ①The diameter and cross-sectional area of longissimus dorsi muscle type Ⅰ, type ⅡA and typeⅡB of adult cows were higher than those of calves, but there was no significant difference in the diameter of muscle fiber of typeⅡB(P>0.05), while the muscle fiber density of typeⅠ, type ⅡA and type ⅡB of calves was significantly higher than that of adult calves (P<0.05). ②The calf MyHCⅠ and MyHCⅡa gene mRNA expression of adult cattle was significantly higher than those of calves (P<0.05), while MyHCⅡb and MyHCⅡx gene mRNA expression presented contrary trend (P<0.05). ③The total antioxidant capacity (T AOC) and superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) and malondialdehyde (MDA), and other indicators of muscles showed no significant difference between two groups (P>0.05). 【Conclusion】 Above all, the oxidizing muscle fiber types and meat quality of yak were decreased with age, while the antioxidant capacity not obviously increased at the same time.

Key words: Bos grunniens, histological characteristics, myosin heavy chain, ATPase staining, antioxidant index

中图分类号:

S823.85

周力, 姚有莉, 李蒋伟, 马博妍, 高占红, 桂林生. 幼龄和成年牦牛肌纤维类型、MyHC基因表达及其肌肉抗氧化能力的比较研究[J]. 四川农业大学学报, 2021, 39(2): 254-259.

ZHOU Li, YAO YouLi, LI Jiangwei, MA Boyan, GAO Zhanhong, GUI Linsheng. Comparative Study on Muscle Fiber Types, MyHC Gene Expression and Antioxidant Capacity of Yak at Different Developmental Stages[J]. Journal of Sichuan Agricultural University, 2021, 39(2): 254-259.

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