四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (2): 240-247.doi: 10.16036/j.issn.1000-2650.2021.02.015

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饲粮中不同比例玉米皮对生长肉兔肠道形态、消化酶活性、抗氧化能力、免疫功能及盲肠发酵参数的影响

蔡景义1,2#, 曾绘锦1#, 曾亭轩1, 焦艳娜1, 贾刚1,2, 赵华1,2, 田刚1,2,*   

  1. 1.四川农业大学动物营养研究所,成都 611130;
    2.动物抗病营养教育部重点实验室,成都 611130
  • 收稿日期:2020-10-20 出版日期:2021-04-28 发布日期:2021-05-07
  • 通讯作者: * 田刚,教授,E-mail:tgang2008@126.com。
  • 作者简介:蔡景义,副教授,博士,从事饲料资源开发及高效利用研究。#同等贡献作者。
  • 基金资助:
    成都市农业技术研发项目(2015-NY02-00271-NC); 四川省科技支撑计划项目(2016NZ0002)

Effects of Different Proportions of Corn Bran in Diets on Intestinal Morphology, Digestive Enzyme Activities, Antioxidant Capacity, Immunity and Cecal Fermentation Parameters of Growing Rabbits

CAI Jingyi1,2#, ZENG Huijin1#, ZENG Tingxuan1, JIAO Yanna1, JIA Gang1,2, ZHAO Hua1,2, TIAN Gang1,2,*   

  1. 1. Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130;
    2. Key Laboratory of Animal Disease-Resistant Nutrition,Ministry of Education,Chengdu 611130
  • Received:2020-10-20 Online:2021-04-28 Published:2021-05-07

摘要: 【目的】旨在考察饲粮中不同比例玉米皮对生长肉兔肠道形态、消化酶活性、抗氧化能力、免疫功能及盲肠发酵参数的影响。【方法】选取150只体重相近、健康的35日龄断奶商品新西兰白兔,随机分为5组,每组15个重复,每个重复2只。对照组(C组)和4个试验组(T1~T4组)分别饲喂5种含0%、5%、10%、15%和20%玉米皮的等能等氮等纤维饲粮。试验期14 d。【结果】①玉米皮饲粮组十二指肠绒毛高度和绒毛高度与隐窝深度比值均高于对照组,其中T3和T4组达到极显著水平(P<0.01),其他小肠形态结构指标差异不显著(P>0.05)。②玉米皮饲粮组空肠淀粉酶活性均高于对照组,其中T4组达到显著水平(P<0.05);T1和T4组回肠胰蛋白酶活性高于对照组,其中T1组达到显著水平(P<0.05);其他肠道消化酶活性无显著差异(P>0.05)。③玉米皮饲粮组回肠总超氧化物歧化酶(T-SOD)活性均高于对照组,其中T2和T4组达到显著水平(P<0.05),其他肠道抗氧化指标差异不显著(P>0.05)。④T1组回肠MUC2含量显著高于其他各组(P<0.05),而其他肠道免疫指标无显著差异(P>0.05)。⑤除T1组外,玉米皮饲粮组总挥发性脂肪酸(VFA)浓度、乙酸比例和乙酸/丙酸比值高于对照组,丙酸比例和丁酸比例低于对照组。【结论】在本试验条件下,饲粮中不同比例玉米皮对生长肉兔肠道形态、消化酶活性、抗氧化能力、免疫功能及盲肠发酵功能无明显不良影响;结合生产实际及前期研究中生长性能和经济效益,推荐生长肉兔饲粮中玉米皮的适宜用量为15%(w/w)。

关键词: 玉米皮, 生长肉兔, 消化酶活性, 抗氧化能力, 盲肠发酵参数

Abstract: 【Objective】 This study was aimed to determine the effects of different proportions of corn bran in diets on intestinal morphology, intestinal enzyme activities, antioxidant capacity, immunity and cecal fermentation parameters of growing rabbits. 【Method】 150 weaned New Zealand white rabbits, aged 35 days with similar body weight were randomly divided into 5 groups (15 replicates/group, 2 rabbits/ replicate). The rabbits in control group (group C) and 4 experimental groups (T1 group, T2 group, T3 group and T4 group) were fed five isoenergetic, isonitrogenous and isofibrous diets containing 0%, 5%, 10%, 15% and 20% corn bran, respectively. The experiment period was 14 days. 【Result】 The results showed that: ①The duodenal villi height and the ratio of the height of the villi to the depth of the crypt of the corn bran diet groups were higher than those of the control group, among which T3 group and T4 group were significantly higher than those of the control group (P<0.01), and the differences among other small intestine morphological and structural indicators were not significant (P>0.05). ② The activity of jejunal amylase in the corn bran diet groups was higher than that in the control group, and the T4 group was significantly higher than that in the control group (P<0.05). The ileal trypsin activity of T1 and T4 groups was higher than that of the control group, and the difference was significant in T1 group (P<0.05). There was no significant difference in the activity of other intestinal digestive enzymes between the groups (P>0.05). ③ The T-SOD activity of the corn bran diet groups was higher than that of the control group, and the T2 group and T4 group were significantly higher than that of the control group (P<0.05), while the difference of other intestinal antioxidant indexes between the groups was not significant (P>0.05). ④The content of ileum MUC2 in T1 group was significantly higher than that in other groups (P<0.05), while there was no significant difference among other intestinal immunity indicators (P>0.05). ⑤Except the T1 group, the TVFA,acetic acid ratio and acetic acid/propionic acid in the corn bran diet group were all higher than the control group, while the propionic acid ratio and butyric acid ratio were lower than the control group. 【Conclusion】Under the conditions of this experiment, different proportions of corn bran in diets had no negative effects on intestinal morphology, digestive enzyme activities, antioxidant capacity, immunity and cecal fermentation parameters of growing rabbits, based on the actual production and growth performance and economic efficiency in the earlier study, the appropriate amount of corn bran in the diet of growing rabbit is 15% (w/w).

Key words: corn bran, growing rabbits, digestive enzyme activities, antioxidant capacity, cecal fermentation parameters

中图分类号: 

  • S816