甘薯基因组BBX转录因子基因鉴定与逆境胁迫表达分析

doi:10.16036/j.issn.1000-2650.2021.02.004

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (2): 157-165.doi: 10.16036/j.issn.1000-2650.2021.02.004

甘薯基因组BBX转录因子基因鉴定与逆境胁迫表达分析

毕楚韵^1,2, 黄小芳^1,2, 黄伟群³, 刘江洪¹, 胡韵卓¹, 黄碧芳⁴, 林世强^1,4,*, 陈选阳^1,2,5,*

1.福建农林大学作物生物技术福建省高校重点实验室,福州 350002;
2.福建农林大学农学院, 福州 350002;
3.福建省种子总站,福州 350003;
4.福建农林大学生命科学学院,福州 350002;
5.福建农林大学教育部作物遗传育种与综合利用重点实验室,福州 350002

收稿日期:2020-12-17 出版日期:2021-04-28 发布日期:2021-05-07
通讯作者: * 陈选阳,博士,研究员,主要从事甘薯遗传育种和生物信息学研究,E-mail: cxy@fafu.edu.cn;林世强,博士,讲师,主要从事甘薯遗传育种和生物信息学研究,E-mail：linshiqiang@fafu.edu.cn。
作者简介:毕楚韵,硕士研究生。
基金资助:
福建省科技厅项目（2019N0050）

Identification of BBX Transcription Factor Genes in Ipomoea batatas Genome and Expression Analysis under Stresses

BI Chuyun^1,2, HUANG Xiaofang^1,2, HUANG Weiqun³, LIU Jianghong¹, HU Yunzhuo¹, HUANG Bifang⁴, LIN Shiqiang^1,4,*, CHEN Xuanyang^1,2,5,*

1. Fujian Agriculture and Forestry University,Key Laboratory of Crop Biotechnology,Fujian Province Universities,Fuzhou 350002,China;
2. Fujian Agriculture and Forestry University,College of Agriculture,Fuzhou 350002,China;
3. Seed Centre of Fujian,Fuzhou 350003,China;
4. Fujian Agriculture and Forestry University,College of Life Science,Fuzhou 350002,China;
5. Fujian Agriculture and Forestry University,Key Lab of Genetics,Breeding and Multiple Application of Crops,Ministry of Education,Fuzhou 350002,China

Received:2020-12-17 Online:2021-04-28 Published:2021-05-07

摘要/Abstract

摘要： 【目的】甘薯BBX基因的鉴定和逆境胁迫表达分析可为探究其功能和甘薯的抗性育种提供参考。【方法】利用甘薯栽培种“泰中6号”全基因组序列和甘薯逆境胁迫下的转录组数据,进行BBX转录因子的鉴定和分析,包括进化树、染色体定位、基因相似度分析、保守结构域、保守基序和抗逆表达模式。【结果】甘薯基因组中共鉴定到24个BBX转录因子基因,其中5对基因相似度高。BBX基因编码的蛋白具有1个或2个B-Box结构域,其中8个蛋白还包含CCT结构域,这些结构域在甘薯BBX蛋白中均较为保守,保守基序motif 2代表甘薯BBX蛋白的CCT结构域。转录组学数据结果表明,甘薯在蔓割病菌侵染后,有3个BBX基因发生差异性表达。甘薯块根在长达2周或6周的冷胁迫下,13个BBX基因表达量发生变化,其中多数为表达下调。【结论】甘薯全基因组中鉴定得到24个BBX转录因子基因,在低温胁迫和蔓割病胁迫条件下分别有13和3个基因存在差异表达。

关键词: 甘薯, BBX, 转录因子, 生物信息学

Abstract: 【Objective】 The identification of Ipomoea batatas BBX genes and the expression analysis under stresses could provide reference for the functional study on I. batatas BBX genes and for the resistance breeding of I. batatas. 【Method】 In this study, the genome sequence of the I. batatas cultivar ‘Taizhong 6’ and the transcriptomics data of I. batatas under stresses were utilized to conduct the identification and analysis on the BBX transcription factors, which included phylogenetics, chromosomal localization, analysis of gene similarity, conservative domains, conservative motifs and expression patterns under stresses. 【Result】 A total of 24 BBX transcription factor genes were identified in the I. batatas genome, 5 pairs of which showed a high similarity. These BBX proteins contained 1 or 2 B-Box domains and 8 of them also had CCT domain. The B-Box and CCT domains were relatively conserved in I. batatas BBX proteins and the conservative motif 2 represented the CCT domain of I. batatas BBX proteins. Results of transcriptomic data showed that there were 3 BBX genes differentially expressed in I. batatas after Fusarium oxysporum f. sp. batatas infection and that there were 13 BBX genes differentially expressed in I. batatas tuber under cold stress lasting 2 or 6 weeks, most of which were down-regulated. 【Conclusion】 There were 24 BBX genes identified in the whole genome of sweet potato. There were 13 and 3 I. batatas BBX genes expressed differentially under the stress of F. oxysporum f. sp. batatas and under low temperature stress, respectively.

Key words: Ipomoea batatas, BBX, transcription factor, bioinformatics

中图分类号:

S531

毕楚韵, 黄小芳, 黄伟群, 刘江洪, 胡韵卓, 黄碧芳, 林世强, 陈选阳. 甘薯基因组BBX转录因子基因鉴定与逆境胁迫表达分析[J]. 四川农业大学学报, 2021, 39(2): 157-165.

BI Chuyun, HUANG Xiaofang, HUANG Weiqun, LIU Jianghong, HU Yunzhuo, HUANG Bifang, LIN Shiqiang, CHEN Xuanyang. Identification of BBX Transcription Factor Genes in Ipomoea batatas Genome and Expression Analysis under Stresses[J]. Journal of Sichuan Agricultural University, 2021, 39(2): 157-165.

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甘薯基因组BBX转录因子基因鉴定与逆境胁迫表达分析

Identification of BBX Transcription Factor Genes in Ipomoea batatas Genome and Expression Analysis under Stresses

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