通过抑制水稻miR168改良产量、抗性和生育期

doi:10.16036/j.issn.1000-2650.2021.02.001

四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (2): 137-140.doi: 10.16036/j.issn.1000-2650.2021.02.001

• • 下一篇

通过抑制水稻miR168改良产量、抗性和生育期

李燕, 王贺, 朱勇, 张凌荔, 鲁均华, 王文明^*

四川农业大学西南作物基因资源发掘与利用国家重点实验室,成都 611130

收稿日期:2021-03-24 出版日期:2021-04-28 发布日期:2021-05-07
通讯作者: * 王文明,教授,主要从事水稻抗病机制与应用研究,E-mail：j316wenmingwang@sicau.edu.cn。
作者简介:李燕,副教授,主要从事水稻抗病机制及应用研究,E-mail：jiazaihy@163.com。
基金资助:
国家自然科学基金项目（U19A2033、31672090和31430072）; NIH基金项目（GM59962）; USDA NIFA基金项目（2017-67013-26590）; Joint BioEnergy Institute 基金项目（DE-AC02-05CH11231）

Suppression of Rice miR168 Improves Yield, Flowering Time and Immunity

LI Yan, WANG He, ZHU Yong, ZHANG Lingli, LU Junhua, WANG Wenming^*

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,China

Received:2021-03-24 Online:2021-04-28 Published:2021-05-07

摘要/Abstract

摘要： 【目的】作物产量、生育期和抗性是生产中的3个关键因素。提高抗性往往影响产量,而高产往往伴随更长的生育期。发掘能协调这3个因素的基因,对作物高产抗病研究和育种具有重大意义。【方法】利用遗传、生化和植物病理学等技术手段,对水稻miR168调控水稻产量、生育期和抗性的功能和相关机制进行了分析。【结果】过表达miR168导致稻瘟病抗性降低,植株变高,分蘖减少,产量降低,开花延迟;而表达miR168的一个模拟靶标（target mimic,MIM168）从而抑制miR168的功能后,稻瘟病抗性增强,植株变矮,分蘖和产量增加,花期提前。发现miR168影响整个miRNA网络,通过miR1320调控稻瘟病抗性,通过miR535平衡分蘖发育和稻瘟病抗性,通过miR164控制开花时间和稻瘟病抗性。相关研究成果以"Suppression of rice miR168 improves yield, flowering time and immunity"为题于2021年2月发表在国际期刊Nature Plants（doi.org/10.1038/s41477-021-00852-x）。【结论】成果揭示了单个miRNA可协同调控水稻产量、生育期和稻瘟病抗性。相关研究成果为水稻高产高抗病育种提供了备选基因资源。

关键词: 水稻, miR168, AGO1, 稻瘟病抗性, 产量, 生长周期

Abstract: 【Objective】 Yield, flowering time, and disease resistance are key factors in rice production. However, the presence of disease resistance gene can penalize crop yield. Looking for genes that can coordinate the three factors is helpful for rice immunity research and breeding. 【Method】 We adopt various techniques in genetics, biochemistry, and plant pathology to dissect the function and mechanism of miR168 in rice yield, growth period and blast disease resistance. 【Result】 Our results demonstrate that suppression of miR168 by a target mimic (MIM168) not only improves grain yield and shortens flowering time in rice, but also enhances immunity to M. oryzae. These results were validated through repeated tests in rice fields in the absence and presence of rice blast pressure. We found that miR168-AGO1 module regulates miR535 to improve yield by increasing panicle number, miR164 to reduce flowering time, and miR1320 and miR164 to enhance immunity. The related results were Published on Nature Plants in February, 2021 with the title “Suppression of rice miR168 improves yield, flowering time and immunity” (doi.org/10.1038/s41477-021-00852-x). 【Conclusion】 Our discovery demonstrated that changes in a single miRNA enhance the expression of multiple agronomically important traits.

Key words: rice, miR168, AGO1, blast disease resistance, yield, growth period

中图分类号:

S435.111.1

李燕, 王贺, 朱勇, 张凌荔, 鲁均华, 王文明. 通过抑制水稻miR168改良产量、抗性和生育期[J]. 四川农业大学学报, 2021, 39(2): 137-140.

LI Yan, WANG He, ZHU Yong, ZHANG Lingli, LU Junhua, WANG Wenming. Suppression of Rice miR168 Improves Yield, Flowering Time and Immunity[J]. Journal of Sichuan Agricultural University, 2021, 39(2): 137-140.

参考文献

[1] BERGELSON J, PURRINGTON C B.Surveying patterns in the cost of resistance in plants[J]. Am Nat, 1996, 148: 536-558.
[2] NELSON R, WIESNER-HANKS T, WISSER R, et al.Navigating complexity to breed disease-resistant crops[J]. Nat Rev Genet,2018, 19: 21-33.
[3] EVANS J R.Improving photosynthesis[J]. Plant Physiol, 2013, 162: 1780-1793.
[4] ROSSI M, BERMUDEZ L, CARRARI F.Crop yield: challenges from a metabolic perspective[J]. Curr Opin Plant Biol, 2015, 25:79-89.
[5] CHANDRAN V, WANG H, GAO F, et al.miR396-OsGRFs module balances growth and rice blast disease-resistance[J]. Front Plant Sci, 2019, 9: 1999.
[6] TANG J Y, CHU C C.MicroRNAs in crop improvement: fine-tuners for complex traits[J]. Nat Plants, 2017, 3(7): 1-11.
[7] LI Y, LU Y G, SHI Y, et al.Multiple rice microRNAs are involved in immunity against the blast fungus Magnaporthe oryzae[J]. Plant Physiol, 2014, 164: 1077-1092.
[8] SUN M Z, SHEN Y, LI H Y, et al.The multiple roles of OsmiR535 in modulating plant height, panicle branching and grain shape[J]. Plant Science, 2019, 283: 60-69.
[9] YANG R X, LI P C, MEI H L, et al.Fine-tuning of miR528 accumulation modulates flowering time in rice[J]. Mol Plant, 2019, 12(8): 1103-1113.
[10] ZHAO Y F, PENG T, SUN H Z, et al.miR1432-OsACOT (Acyl-CoA thioesterase) module determines grain yield via enhancing grain filling rate in rice[J]. Plant Biotechnol J,2019,17: 712-723.
[11] LI Y, CAO X L, ZHU Y, et al.Osa-miR398b boosts H₂O₂ production and rice blast disease-resistance via multiple superoxide dismutases[J]. New Phytol, 2019, 222: 1507-1522.
[12] LI Y, ZHAO S L, LI J L, et al.Osa-miR169 negatively regulates rice immunity against the blast fungus Magnaporthe oryzae[J]. Front Plant Sci, 2017, 8(6): 2.
[13] SALVADOR-GUIRAO R, HSING Y I, SAN SEGUNDO B.The Polycistronic miR166k-166h positively regulates rice immunity via post-transcriptional control of EIN2[J]. Front Plant Sci, 2018, 9: 337.
[14] XIAO Z Y, WANG Q X, ZHAO S L, et al.Mir444 b.2 regulates resistance to Magnaporthe oryzae and tillering in rice[J]. Acta Phytppathol Sin, 2017, 47: 511-522.
[15] ZHANG X, BAO Y L, SHAN D Q, et al.Magnaporthe oryzae Induces the expression of a MicroRNA to suppress the immune response in rice[J]. Plant Physiol, 2018, 177: 352-368.
[16] ZHAO Z X, FENG Q, CAO X L, et al.Osa-miR167d facilitates infection of Magnaporthe oryzae in rice[J]. J Integr Plant Biol, 2019,62: 702-715.
[17] JIAO Y Q, WANG Y H, XUE D W, et al.Regulation of OsSPL14 by OsmiR156 defines ideal plant architecture in rice[J]. Nat Genet, 2010, 42: 541-544.
[18] LEI W, SUN S, JIN J, et al.Coordinated regulation of vegetative and reproductive branching in rice[J]. Proc Natl Acad Sci U S A, 2015, 112(50): 15504-15509.
[19] MANNAI Y E, AKABANE K, HIRATSU K, et al.The NAC transcription factor gene OsY37 (ONAC011) promotes leaf senescence and accelerates heading time in rice[J]. International Journal of MolecularSciences, 2017, 18: 2165-2185.

通过抑制水稻miR168改良产量、抗性和生育期

Suppression of Rice miR168 Improves Yield, Flowering Time and Immunity

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10

[1]	钦鹏, 陈薇兰, 王淏, 李仕贵. 基于33个遗传多样性水稻材料的泛基因组分析揭示“隐藏”的基因组变异[J]. 四川农业大学学报, 2021, 39(3): 275-278.
[2]	李丽, 赵彦丰, 吴琪琦, 黄燕. 利用R基因RRS1/RPS4探索水稻原生质体瞬时表达转化体系的研究[J]. 四川农业大学学报, 2021, 39(3): 297-306.
[3]	刘唯佳, 毛昆明, 唐祺超, 杨元智, 邓良基. 菌渣替代部分化肥养分施用对土壤养分含量及稻麦产量的影响[J]. 四川农业大学学报, 2021, 39(3): 323-330.
[4]	邓达行, 陈亭微, 陈雨轩, 罗玲. 磷酸改性水稻秸秆和猪粪生物质炭添加对不同土壤吸附四环素的影响[J]. 四川农业大学学报, 2021, 39(3): 331-340.
[5]	钦鹏, 樊世军, 高鹏, 李仕贵. 水稻耐高温相关基因功能及其信号通路研究进展[J]. 四川农业大学学报, 2021, 39(3): 279-285.
[6]	吕腾飞, 谌洁, 代邹, 杨志远, 郑传刚, 马均. 氮肥缓速配施对两种机插稻物质生产特性的影响[J]. 四川农业大学学报, 2021, 39(3): 286-296.
[7]	蒋波, 孙加威, 何舜, 任哓波, 冯生强, 阎洪, 林金平. 2009—2019年成都市中籼迟熟水稻品种产量构成分析[J]. 四川农业大学学报, 2021, 39(3): 307-315.
[8]	胡雨寒, 张荣萍, 陶诗顺, 侯永康, 黄正, 张琪, 周宁宁, 阿什日轨. 川北丘陵区水稻油后直播品种适应性研究[J]. 四川农业大学学报, 2021, 39(3): 316-322.
[9]	靳亚忠, 熊亚男, 孙雪, 李金雨, 李响, 何淑平, 李春霞. 化肥减施与木霉菌有机肥配施对辣椒产量、品质及根际土壤酶活性的影响[J]. 四川农业大学学报, 2021, 39(2): 198-204.
[10]	钦鹏, 张国华, 胡彬华, 李仕贵. 脱落酸长距离运输的生物学意义和分子机制解析[J]. 四川农业大学学报, 2021, 39(1): 1-3.
[11]	蒋明金, 大川泰一郎, 马均. 播栽方式对2个籼稻品种抗倒伏能力的影响[J]. 四川农业大学学报, 2020, 38(04): 391-398.
[12]	蒋明金, 李敏, 罗德强, 周维佳, 江学海, 姬广梅, 李立江. 播种量对不同粒型机插优质杂交籼稻秧苗素质、机插质量及产量的影响[J]. 四川农业大学学报, 2020, 38(04): 399-408.
[13]	代顺冬, 韦树谷, 赖佳, 黄玲, 张骞方, 叶鹏盛, 盛玉珍, 刘佳. 水、氮、磷、钾耦合效应对大白菜产量的影响[J]. 四川农业大学学报, 2020, 38(03): 299-304.
[14]	戴晓康, 冯茂松, 黄从德, 毕浩杰, 赖娟, 刘雄, 高嘉翔, 白文玉. 四川盆地南缘不同海拔金佛山方竹林（Chimonobambusautilis）土壤化学计量及笋产量特征[J]. 四川农业大学学报, 2020, 38(01): 43-51.
[15]	张巫军, 段秀建, 姚雄, 唐永群, 杨小艳, 刘强明, 肖人鹏, 张现伟, 李经勇. 烯效唑对遮阴下重穗型水稻渝香203茎秆形态结构和抗倒伏性的影响[J]. 四川农业大学学报, 2019, 37(06): 755-761.