四川农业大学学报 ›› 2021, Vol. 39 ›› Issue (1): 19-26.doi: 10.16036/j.issn.1000-2650.2021.01.004

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黄瓜CDPK基因家族的鉴定与进化特征分析

靳燕, 王谢琴, 卿华, 马政, 姚慧鹏*   

  1. 四川农业大学生命科学学院,四川 雅安 625014
  • 收稿日期:2020-07-16 出版日期:2021-02-28 发布日期:2021-03-05
  • 作者简介:靳燕,硕士研究生。*责任作者:姚慧鹏,博士,副教授,主要从事病毒、植物、动物密码子及进化生物学等相关生物信息学研究,E-mail:yaohuipeng0921@163.com。
  • 基金资助:
    四川农业大学2020年学科建设双支计划(2021993090)

Identification and Evolutionary Characteristics of CDPK Gene Family in Cucumber

JIN Yan, WANG Xieqin, QING Hua, MA Zheng, YAO Huipeng*   

  1. College of Life Sciences,Sichuan Agricultural University,Ya'an 625014,Sichuan,China
  • Received:2020-07-16 Online:2021-02-28 Published:2021-03-05

摘要: 【目的】对黄瓜基因组中CDPK基因的鉴定和进化特征的分析,为深入探索黄瓜CDPK蛋白生物学功能提供理论依据。【方法】用生物信息学方法,在黄瓜的全基因组范围内鉴定CDPK基因,并分析该基因的结构、染色体分布、进化关系及功能域。【结果】在黄瓜全基因组库中总共鉴定出17个CsCPKs,被分为4类;所有CsCPKs均含有6~8个内含子且都具有motif1~10,motif12和motif14;所编码氨基酸序列中含有4个 EF-手型结构;多数CsCPKs具有棕榈酰化位点。【结论】CsCPKs都具有ATP结合、Ca2+结合和蛋白激酶活性的分子功能,参与蛋白质磷酸化的生物过程,而特有的motif决定了某些CsCPKs特有的生物学功能。

关键词: 黄瓜, 钙依赖性蛋白激酶, 全基因组分析, 蛋白质结构域预测

Abstract: 【Objective】 We want to identify and analyze the evolutionary characteristics of the CDPK gene in cucumber genome to provide a theoretical basis for in-depth exploration of the biological function of cucumber CDPK protein.【Method】 Bioinformatics was used to identify the CDPK gene within the genome-wide of cucumber,and to analyze its structure,chromosome distribution,evolutionary relationship and functional domain prediction.【Result】 A total of 17 CsCPKs,identified in the cucumber genome-wide library were classified into four categories;all CsCPKs contain 6-8 introns and all have motif1~10,motif12 and motif14;the encoded amino acid sequence contains four hand-type structures;most CsCPKs have palmitoylation sites.【Conclusion】 CsCPKs are produced by three large genome-wide replication events,not caused by heterologous polyploidization events,and the emergence of CsCPKs occurred at least 100 million years ago.All of CsCPKs have the molecular functions of ATP binding,Ca2+ binding,protein kinase activity,and participate in the biological process of protein phosphorylation,and the unique motif determines the specific biological functions of some CsCPKs.

Key words: cucumber, calcium-dependent protein kinase, genome-wide analysis, protein domain prediction

中图分类号: 

  • S642.2