关键词: 黄瓜, 钙依赖性蛋白激酶, 全基因组分析, 蛋白质结构域预测

Abstract: 【Objective】 We want to identify and analyze the evolutionary characteristics of the CDPK gene in cucumber genome to provide a theoretical basis for in-depth exploration of the biological function of cucumber CDPK protein.【Method】 Bioinformatics was used to identify the CDPK gene within the genome-wide of cucumber,and to analyze its structure,chromosome distribution,evolutionary relationship and functional domain prediction.【Result】 A total of 17 CsCPKs,identified in the cucumber genome-wide library were classified into four categories;all CsCPKs contain 6-8 introns and all have motif1~10,motif12 and motif14;the encoded amino acid sequence contains four hand-type structures;most CsCPKs have palmitoylation sites.【Conclusion】 CsCPKs are produced by three large genome-wide replication events,not caused by heterologous polyploidization events,and the emergence of CsCPKs occurred at least 100 million years ago.All of CsCPKs have the molecular functions of ATP binding,Ca²⁺ binding,protein kinase activity,and participate in the biological process of protein phosphorylation,and the unique motif determines the specific biological functions of some CsCPKs.

Key words: cucumber, calcium-dependent protein kinase, genome-wide analysis, protein domain prediction