大豆品种对间作玉米苗期根系生长及抗氧化特性的影响

doi:10.16036/j.issn.1000-2650.2021.01.003

摘要/Abstract

摘要： 【目的】为探究不同大豆品种与种植模式对苗期玉米根系的影响,揭示玉米大豆间套作对玉米根系发育的影响。【方法】以46种大豆材料与玉米进行间作,对苗期玉米的根系形态、活力及SOD抗氧化酶活性进行聚类分析。在阈值为8.5时,将供试大豆材料划分为3个类群。不同大豆品种对间作玉米苗期的影响不同,类群Ⅰ强于类群Ⅱ、Ⅲ,结合四川省主推品种类型,从类群Ⅰ中选择3个大豆品种与玉米进行间作,进一步分析不同种植模式下玉米生长及其根系形态、生理指标的变化。【结果】同单作玉米相比,与“南豆25”“桂夏1号”和“贡秋豆4号”间作条件下,间作玉米地上部分生物量、根系生物量显著提高,地上部分生物量分别比单作玉米高56.4%、80.5%和32.6%,根系生物量分别比单作玉米高38.2%、57.4%和24.7%;间作玉米苗期根系长度、根系表面积显著提高;间作玉米苗期根系活力、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性显著提高,根系活力分别比单作玉米高27.7%、25.0%和39.7%;SOD活性分别高97.4%、71.2%和120.9%;CAT活性分别高47.4%、67.5%和44.7%;POD活性分别高28.5%、11.8%和16.2%;膜脂过氧化产物丙二醛(MDA)含量显著低于单作。【结论】种植模式对玉米根系生长影响显著,玉米-大豆间作种植可以促进玉米苗期根系生长,延缓玉米根系衰老;“南豆25”对间作玉米苗期根系影响小于“桂夏1号”“贡秋豆4号”。

关键词: 玉米, 根系生长, 间作, 抗氧化酶, 大豆品种

Abstract: 【Objective】 In order to explore the effects of different soybean varieties and planting patterns on maize root system at seedling stage,the effects of maize soybean intercropping on maize root development were revealed.【Method】 46 soybean materials were intercropped with maize.The root morphology,activity and SOD antioxidant enzyme activity of maize at seedling stage were analyzed by cluster analysis.At the threshold of 8.5,the soybean materials were divided into three groups.Different soybean varieties have different effects on the seedling stage of intercropping maize.Group I is stronger than group Ⅱ and Ⅲ.Combining with the main types of varieties promoted in Sichuan province,three soybean varieties were selected from group I and intercropped with maize,and the changes of maize growth,root mor-phology and physiological indexes under different planting modes were further analyzed.【Result】 Compared with the monocropped maize,the aboveground biomass and root biomass of the intercropped system for ‘Nandou25’‘Guixia 1’and‘Gongqiudou 4’ were significantly increased by 56.4%,80.5% and 32.6%.Root biomass of the intercropped for three varieties were significantly increased by 38.2%,57.4% and 24.7%,respectively.Compared with the monocropped maize,the root length of the intercropped system for three varieties at seedling stage was significantly increased by 27.7%,25.0% and 39.7%.The activities of root system,super oxide dismutase (SOD),catalase (CAT) and peroxidase (POD) in intercropping system for three varieties were significantly increased by 27.7%,25.0% and 39.7%,97.4%,71.2% and 120.9%,47.4%,67.5% and 44.7%,28.7%,11.8% and 16.2%,respectively.The content of malondialdehyde (MDA) in intercropping system for three varieties was significantly decreased than the monocropped maize.【Conclusion】 The effect of planting mode on the root growth of maize is significant.The intercropping of maize and soybean can promote the root growth of maize at seedling stage and delay the senescence of maize root; the effect of ‘Nandou 25’ on the root of maize at seedling stage is less than‘Guixia 1’and‘Gongqiudou 4’.

Key words: maize, root growth, intercropping, antioxidant enzymes, soybean varieties

中图分类号:

S513

杨雪丽, 吴吉英子, 刘凡, 刘旭东, 任俊波, 杜青, 陈平, 雍太文. 大豆品种对间作玉米苗期根系生长及抗氧化特性的影响[J]. 四川农业大学学报, 2021, 39(1): 11-18.

YANG Xueli, WU Jiyingzi, LIU Fan, LIU Xudong, REN Junbo, DU Qing, CHEN Ping, YONG Taiwen. Effects of Different Soybean Varieties on Root Growth and Physiological Characteristics of Intercropping Maize[J]. Journal of Sichuan Agricultural University, 2021, 39(1): 11-18.

参考文献

[1] 王琦琪,陈印军.我国玉米种植的优势分析[J].中国农业科技导报,2018,220(3):1-9.
[2] 李锋.2020年中国和美国玉米生产成本差异预估及技术需求预估分析[J].安徽农业科学,2019,47(15):220-223.
[3] 刘金云.浅析新技术对提高玉米种植效率的作用[J].种子科技,2019(8):60-61.
[4] 徐春丽,谢军,王珂,等.中国西南地区玉米产量对基础地力和施肥的响应[J].中国农业科学,2018,51(1):129-138.
[5] 雍太文,任万军,杨文钰,等.旱地新3熟“麦-玉-豆”模式的内涵的特点及栽培技术[J].耕作与栽培,2006,48(6):48-50.
[6] 雍太文,杨文钰,向达兵,等.小麦/玉米/大豆套作的产量、氮营养表现及其种间竞争力的评定[J].草业学报,2012,21(1):50-58.
[7] 解占军,汪仁,安景文,等.不同施肥技术对防止玉米早衰及对产量的影响[J].辽宁农业科学,2008(6):29-31.
[8] 李彩虹,吴伯志.玉米间套作种植方式研究综述[J].玉米科学,2005,13(2):85-89.
[9] ZHOU T,WANG L,DU Y,et al.Rhizosphere soil bacterial community composition in soybean genotypes and feedback to soil P availability[J].Journal of Integrative Agriculture,2019,18(10):2230-2241.
[10] 雍太文,杨文钰,任万军,等.发展套作大豆促进四川大豆产业发展[J].作物杂志,2007(6):5-8.
[11] 管建慧,郭新宇,王纪华,等.玉米不同部位根系生长发育规律的研究[J].玉米科学,2007,15(6):82-85.
[12] 董学会,段留生,何钟佩,等.30%己乙水剂对玉米根系伤流液及其组分的影响[J].西北植物学报,2005,25(3):587-591.
[13] 王玉贞,李维岳,尹枝瑞.玉米根系与产量关系的研究进展[J].吉林农业科学,1999,24(4):6-8.
[14] 鱼欢,冯佰利,张英,等.不同栽培模式下冬小麦叶片衰老与活性氧代谢研究[J].作物学报,2007,33(10):1729-1732.
[15] 张含彬,伍晓燕,杨文钰,等.氮肥对套作大豆干物质积累与分配的影响[J].大豆科学,2006,25(4):404.
[16] 管建慧.玉米根系生长发育特性及与地上部关系的研究[D].呼和浩特:内蒙古农业大学,2007.
[17] 黄高宝,张恩和.禾本科-豆科作物间套种植对根系活力影响的研究[J].草业学报,1998,7(2):18-22.
[18] 雍太文,杨文钰,向达兵,等.不同种植模式对作物根系生长:产量及根际土壤微生物数量的影响[J].应用生态学报,2012,23(1):125-132.
[19] 杨航.品种与根系分隔对玉米/大豆套作下作物生长、氮素吸收及大豆生物固氮的影响[D].成都:四川农业大学,2016.
[20] 高英波.不同栽培模式下超高产春玉米根冠衰老特性研究[D].呼和浩特:内蒙古农业大学,2011.
[21] 蒋健,王宏伟,刘国玲,等.生物炭对玉米根系特性及产量的影响[J].玉米科学,2015,23(4):62-66.
[22] 宋日,牟瑛,王玉兰,等.玉米、大豆间作对两种作物根系形态特征的影响[J].东北师大学报(自然科学),2002,34(3):83-86.
[23] 陈平,杜青,庞婷,等.根系互作强度对玉米-大豆套作系统下作物根系分布及地上部生长的影响[J].四川农业大学学报,2018,36(1):28-37.
[24] XIA H Y,ZHAO J H,SUN J H,et al.Dynamics of root length and distribution and shoot biomass of maize as affected by intercropping with different companion crops and phosphorus application rates[J].Field Crops Research,2013,150:52-62.
[25] 熊庆娥.植物生理学实验教程[M].成都:四川出版集团·四川科学技术出版社,2003.
[26] 雍太文,刘小明,刘文钰,等.减量施氮对玉米-大豆套作体系中作物产量及养分吸收利用的影响[J].应用生态学报,2014,25(2):474-482.
[27] 斯琴巴特尔,吴红英.不同逆境对玉米幼苗根系活力及硝酸还原酶活性的影响[J].干旱地区农业研究,2001,19(2):67-70.
[28] BECKMAN K B,AMES B N.The free radical theory of aging matures[J].Physiological Reviews,1998,78(2):547.
[29] DEVASAGAYAM T P A,TILAK J C,BOLOOR K K,et al.Free radicals and antioxidants in human health:current status and future prospects[J].The Journal of the Association of Physicians of India,2004,52:794-804.
[30] NAVROT N,ROUHIER N,GELHAYE E,et al.Reactive oxygen species generation and antioxidant systems in plant mitochondria[J].Physiologia Plantarum,2007,129(1):185-195.
[31] 王瑞,马凤鸣,李彩凤,等.低温胁迫对玉米幼苗脯氨酸、丙二醛含量及电导率的影响[J].东北农业大学学报,2008,39(5):20-23.
[32] 余泽高,王红闪.冬小麦抗衰性与丙二醛含量相关性的初步探讨[J].湖北农业科学,2003(2):28-29.
[33] 刘均霞.玉米/大豆间作条件下作物根际养分高效利用机理研究[D].贵阳:贵州大学,2008.
[34] 张顺捷,马凤鸣,王玉波,等.大豆根系分泌物化感作用的初步研究[J].东北农业大学学报,2008,39(10):1-5.
[35] 付学鹏,吴凤芝,吴瑕,等.间套作改善作物矿质营养的机理研究进展[J].植物营养与肥料学报,2016,22(2):525-535.
[36] 雍太文,陈小容,杨文钰,等.小麦/玉米/大豆三熟套作体系中小麦根系分泌特性及氮素吸收研究[J].作物学报,2010,36(3):477-485.