套作大豆根腐病与玉米茎腐病致病镰孢菌的相互侵染关系研究

doi:10.16036/j.issn.1000-2650.2020.05.007

四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (05): 551-557.doi: 10.16036/j.issn.1000-2650.2020.05.007

套作大豆根腐病与玉米茎腐病致病镰孢菌的相互侵染关系研究

李红菊¹, 李晓迪¹, 陈思怡¹, 戴浩¹, 常小丽^1,2,*

1.四川农业大学农学院,四川省作物带状复合种植工程技术研究中心,成都 611130;
2.植物病虫害生物学国家重点实验室,中国农业科学院植物保护研究所,北京 100193

收稿日期:2020-06-12 出版日期:2020-10-28 发布日期:2021-02-05
作者简介:李红菊,硕士研究生。^*责任作者：常小丽,副教授,主要从事作物真菌病害发生规律研究,E-mail：xl_chang14042@sicau.edu.cn。
基金资助:
国家重点研发计划项目（2018YFD0200500）; 国家自然科学基金项目（31801685）; 大豆产业技术体系（CARS-04-PS19）

Infection Relationship of Fusarium spp. Causing Soybean Root Rot and Maize Stalk Rot in Maize-Soybean Relay Strip Intercropping

LI Hongju¹, LI Xiaodi¹, CHEN Siyi¹, DAI Hao¹, CHANG Xiaoli^1,2,*

1. College of Agronomy,Sichuan Agricultural University;Sichuan Engineering Research Center for Crop Strip Intercropping System,Chengdu 611130,China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China

Received:2020-06-12 Online:2020-10-28 Published:2021-02-05

摘要/Abstract

摘要： 【目的】明确玉米-大豆带状套作栽培下大豆根腐病与玉米茎腐病的相互作用关系。【方法】以套作大豆根腐病和玉米茎腐病的致病镰孢菌为供试菌株,采用高粱粒接种法、孢子悬液注射接种法和茎刺伤接种法,检测套作大豆根腐病与玉米茎腐病镰孢菌对两种寄主的相互侵染致病性。【结果】大豆根腐病层生镰孢菌F. proliferatum、禾谷镰孢菌F. graminearum、尖孢镰孢菌F. oxysporum、燕麦镰孢菌F. avenaceum、F. commnue和木贼镰孢菌F. equiseti均能侵染玉米根部,引起幼苗发病,但致病力存在差异,以禾谷镰孢菌的致病力最强,病情指数高达85%,且玉米株高和根鲜重与对照相比显著降低;同时,玉米茎腐病禾谷镰孢菌也能侵染大豆茎秆和根部,导致侵染组织变褐坏死,病情指数达78%以上。【结论】在玉米-大豆带状套作栽培下,以禾谷镰孢菌为代表的镰孢菌作为玉米茎腐病和大豆根腐病的重要致病菌能够相互侵染两种寄主,具有加重套作栽培下两种病害的潜在风险,为该栽培模式下镰孢菌相关病害的综合防治提供理论依据。

关键词: 玉米-大豆带状套作, 大豆根腐病, 玉米茎腐病, 镰孢菌, 致病性

Abstract: 【Objective】 This study aimes to study the pathogen interaction relationship of soybean root rot and maize stalk rot in the maize-soybean relay strip intercropping system. 【Method】 The pathogenicity and cross-infection of Fusarium spp. causing soybean root rot and maize stalk rot on two hosts maize and soybean were evaluated through sorghum seeds inoculation, spore injection inoculation and stalk wounding inoculation, respectively. 【Result】 Six Fusarium spp. causing soybean root rot including F. proliferatum, F. graminearum, F. oxysporum, F. avenaceum, F. commune and F. equiseti were able to infect maize seedlings, but differed in their aggressivness, among which, F. graminearum showed the strongest aggressiveness with significant inhibition on seedling height and root fresh weight. And meanwhile, the cross-infection assay showed that F. graminearum from two hosts was able to infect maize or soybean followed by discolored and brown dead spot in infected tissues and growth inhibition. 【Conclusion】 Fusarium spp., the important pathogens of maize stalk rot and soybean root rot, can infect both hosts under maize-soybean relay strip intercropping, which brings a potential threat that these two diseases caused by Fusarium spp. might become more serious in this cropping pattern. The results provide a theoretical basis for the comprehensive control of Fusarium spp. related diseases under maize-soybean relay strip intercropping.

Key words: maize-soybean strip intercropping, soybean root rot, maize stalk rot, Fusarium spp., cross-pathogenicity

中图分类号:

S435.131

李红菊, 李晓迪, 陈思怡, 戴浩, 常小丽. 套作大豆根腐病与玉米茎腐病致病镰孢菌的相互侵染关系研究[J]. 四川农业大学学报, 2020, 38(05): 551-557.

LI Hongju, LI Xiaodi, CHEN Siyi, DAI Hao, CHANG Xiaoli. Infection Relationship of Fusarium spp. Causing Soybean Root Rot and Maize Stalk Rot in Maize-Soybean Relay Strip Intercropping[J]. Journal of Sichuan Agricultural University, 2020, 38(05): 551-557.

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套作大豆根腐病与玉米茎腐病致病镰孢菌的相互侵染关系研究

Infection Relationship of Fusarium spp. Causing Soybean Root Rot and Maize Stalk Rot in Maize-Soybean Relay Strip Intercropping

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