黄瓜PIP2;4基因生物信息学及干旱胁迫下的诱导表达分析

doi:10.16036/j.issn.1000-2650.2020.05.005

摘要/Abstract

摘要： 【目的】探究黄瓜PIP2;4 （CsPIP2; 4 Gene ID： 101215300）在干旱胁迫响应中的可能功能。【方法】以华北型黄瓜‘9930’为试材,基于CsPIP2; 4为前期研究筛选出的干旱胁迫下显著差异表达蛋白,克隆CsPIP2; 4基因,对其进行生物信息学分析,并利用qRT-PCR检测CsPIP2; 4在干旱下的表达。【结果】生物信息学分析显示,CsPIP2; 4 CDS序列有852 bp,编码283个氨基酸,CsPIP2;4蛋白具有疏水性、无信号肽段、有跨膜结构,是定位在质膜的内在蛋白,以无规则卷曲和α-螺旋结构为主,具有典型的水通道蛋白结构功能域,属于MIP超家族;蛋白质同源比对分析显示,CsPIP2; 4与甜瓜PIP2; 7的亲缘关系最近。qRT-PCR结果表明CsPIP2; 4表达受干旱诱导。【结论】CsPIP2; 4可能是参与黄瓜干旱胁迫应答过程重要基因,为后续鉴定其在黄瓜响应干旱中的功能奠定了基础。

关键词: 黄瓜, PIP2, 4, 生物信息学, 干旱, 表达分析

Abstract: 【Objective】 To explore the possible function of PIP2;4 in cucumber (CsPIP2;4 Gene ID：101215300) involvement in drought stress. 【Method】 Using the North China cucumber 9930 as the test material, based on CsPIP2;4 as the significantly differentially expressed protein selected under the drought stress in the previous study. The CsPIP2;4 gene was cloned and analyzed with bioinformatics, and the expression level under drought-stress was detected by Real-time Quantitative PCR. 【Result】 Bioinfor-matics analysis showed that CDS of the CsPIP2;4 was 852 bp in length, encoded a protein of 288 amino acid residues. CsPIP2;4 has hydrophobic and transmembrane domain, but it doesn't have signal peptide. CsPIP2;4 is located intrinsic protein of plasma membrane. The main secondary structure of CsPIP2; 4 is random curl and α-spiral, and it has typical functional domain of aquaporins and belongs to the major intrinsic protein (MIP) family. The evolutionary analysis showed that CsPIP2;4 was the closest of Cme PIP2-7. The qPCR results showed CsPIP2;4 may be involved in the response related to drought stress. 【Conclusion】 CsPIP2; 4 may be an important gene involved in the response process of cucumber under the drought stress, the study lays the foundation for the further function identification of CsPIP2;4 in response to drought.

Key words: cucumber, PIP2, 4, bioinformatics, drought, expression analysis

中图分类号:

S642.2

陈露倩, 赖梦霞, 柴里昂, 李乐乐, 王璐, 杜长霞, 樊怀福, 朱祝军. 黄瓜PIP2;4基因生物信息学及干旱胁迫下的诱导表达分析[J]. 四川农业大学学报, 2020, 38(05): 538-544.

CHEN Luqian, LAI Mengxia, CHAI Liang, LI Lele, WANG Lu, DU Changxia, FAN Huaifu, ZHU Zhujun. Induced Expression in Drought Stress and Bioinformatic Analysis of PIP2;4 Gene in Cucumber[J]. Journal of Sichuan Agricultural University, 2020, 38(05): 538-544.

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