6个优良杉木家系的生长、光合和氮磷养分利用效率差异

doi:10.16036/j.issn.1000-2650.2020.04.010

摘要/Abstract

摘要： 【目的】生长监测和光合生理机制研究可为杉木优良家系的早期选择和科学评价提供理论依据。【方法】从国家杉木工程中心引进62个第3代杉木半同胞家系建立子代测定林,以本地的1.5代杉木为对照（CK）,以生长优于本地杉木的6个杉木家系为对象,研究它们的生长性状、光合特征及其与氮（N）、磷（P）利用效率的内在关系。【结果】3号家系的株高和地径最大,显著高于CK、26和42号家系（P<0.05）;家系间的最大光合速率（P_max）、表观量子效率（Q）、光呼吸速率（R_d）、光饱和点（L_SP）和补偿点（L_CP）的差异均不显著（P>0.05）,但家系间叶绿素a、b及总叶绿素含量的差异显著,26号家系最高,3号家系最低;家系间N、P养分含量及其光合利用效率的差异不显著。相关分析表明,生长性状与P_max、Q、叶N含量呈显著负相关;具有较高L_SP和较低R_d的家系,生长表现更好。N、P化学计量比特征表明幼龄期杉木的生长主要受N的限制,生长较差的家系采取保守的N、P利用策略。【结论】杉木生长性状不仅与净光合速率有关,更与光合总叶面积紧密相关,生长更好的家系对N、P养分需求量更大。综合来看,3、23和43号家系最为优良,可进一步从中筛选优良单株。

关键词: 杉木, 优良家系, 光合性状, 化学计量特征, 氮磷利用效率

Abstract: 【Objective】 Growth monitoring and studying in photosynthetic physiology of Cunninghamia lanceolata families can provide theoretical basis for early selection and scientific evaluation of superior C. lanceolata families. 【Method】 We introduced a total of 62 third-generation C. lanceolata families from the National C. lanceolata Engineering Center and built a half-sib progeny testing plantation in Sichuan Province, using a local 1.5-generation C. lanceolata family as the control (CK). In our study, six of C. lanceolata families that grew better than CK were selected as materials to detect their differences in growth, photosynthetic capacity, nitrogen (N) and (P) phosphorus content and their use efficiency. 【Result】No. 3 family showed the maximum height and basal diameter, which were significantly higher than those of CK, No. 26 and 42 family. There was no significant difference in light-saturated photosynthetic rate (P_max), apparent quantum efficiency (Q), dark respiration rate (R_d), light saturated point (L_SP), and light compensation point (L_CP) among all families. However, differences in content of chlorophyll a (Chl a), b (Chl b) and total amount of chlorophyll (TC) were significant among the families. The maximum chlorophyll amount (Chl a,, Chl b and TC) was found in leaves of No. 26 family, but the minimum chlorophyll amount (Chl a, Chl b and TC) was detected in leaves of No. 3 family. There was no significant difference in N, P content and their use efficiency among the families. Correlation analysis indicated that height of C. lanceolata families was negatively related with P_max, Q and leaf N content. The families with higher L_SP but lower R_d showed better growth conditions. The stoichiometric ratio of N to P indicated that growth of C. lanceolata was mainly limited by N at the early stage, and families with relatively slower growth rate may adopt conservative N and P use strategies. 【Conclusion】 These results suggested that growth of C. lanceolata is not only related to photosynthetic rate, but also closely to total photosynthetic area of tree crown. In conclusion, No. 3, 23 and 43 families were more promising than other families, and excellent individuals could be further selected from them.

Key words: Cunninghamia lanceolata, family selection, photosynthetic characters, stoichiometric charac-teristics, N and P use efficiency

中图分类号:

S722.5

陈娜, 朱鹏, 陈良华, 胡红玲, 张健, 肖玖金, 杨汉波. 6个优良杉木家系的生长、光合和氮磷养分利用效率差异[J]. 四川农业大学学报, 2020, 38(04): 449-456.

CHEN Na, ZHU Peng, CHEN Lianghua, HU Hongling, ZHANG Jian, XIAO Jiujin, YANG Hanbo. Differences in Growth,Photosynthetic Capacity,N and P Use Efficiency among 6 Superior Cunninghamia lanceolata Families[J]. Journal of Sichuan Agricultural University, 2020, 38(04): 449-456.

参考文献

[1] 康向阳. 林木遗传育种研究进展及展望[J]. 南京林业大学学报(自然科学版), 2020, 44(3): 1-22.
[2] 毕影东, 杨传平. 生物技术在林木遗传育种中的应用[J]. 世界林业研究, 2007, 20(6): 23-28.
[3] 祝列克. 新世纪中国林木遗传育种发展战略[J]. 南京林业大学学报, 2001, 25(1): 3-8.
[4] 申婷婷, 姜静, 刘桂丰, 等. 白桦BpCesA基因的生物信息学及表达分析[J]. 植物研究, 2016, 36(6): 909-916.
[5] 施季森. 迎接21世纪现代林木生物技术育种的挑战[J]. 南京林业大学学报, 2000, 24(1): 4-9.
[6] 李悦, 瞿超, 续九如, 等. 中国大陆林木遗传育种学科1949-2003年的研究历程[J]. 北京林业大学学报, 2005, 27(1): 79-87.
[7] 吴鹏飞, 马祥庆, 蒋建, 等. 闽西不同杉木无性系子代测定林幼林生长的比较[J]. 福建农林大学学报(自然科学版), 2009, 38(4): 371-375.
[8] 张志才. 杉木第2代种子园自由授粉子代测定与选择[J]. 福建林业科技, 2012, 39(2): 9-12, 18.
[9] 郑仁华. 杉木遗传育种研究进展与对策[J]. 世界林业研究,2005, 18(3): 63-65.
[10] 施季森. 福建省杉木遗传改良现状与发展技术对策[J]. 福建林业科技, 1994, 21(3): 28-31.
[11] 郑仁华, 施季森, 苏顺德, 等. 杉木第3代种子园营建技术及应用[J]. 森林与环境学报, 2018, 38(4): 406-413.
[12] 孙鹏, 林夕华. 四川省杉木良种材料应用与推广策略[J]. 四川林业科技, 1998, 19(2): 51-56.
[13] 胡庭兴, 李贤伟, 杨祯禄. 四川盆地丘陵及盆周低山区杉木实生林生长特点及提高产量的途径[J]. 四川林勘设计, 1998, 17(3): 44-49.
[14] 闫小红, 尹建华, 段世华, 等. 四种水稻品种的光合光响应曲线及其模型拟合[J]. 生态学杂志, 2013, 32(3): 604-610.
[15] 陈良华, 曹艺, 杨万勤, 等. 三种园林植物对夜间光照的响应与适应特征[J]. 生态学报, 2017, 37(2): 549-556.
[16] 陈飞宇, 罗天祥, 张林, 等. 江西九连山常绿阔叶林主要树种叶建成消耗的比较[J]. 生态学报, 2006, 26(8): 2485-2493.
[17] 陈良华, 徐睿, 张健, 等. 螯合剂对香樟生理特征和镉积累效率的影响[J]. 云南大学学报(自然科学版), 2016, 38(1): 150-161.
[18] 金辉亮, 苏世平, 李毅. 基于光合参数及渗透调节物质不同家系红砂抗旱性的选择[J]. 干旱区资源与环境, 2017, 31(7): 156-161.
[19] 李茂, 任正标, 郑鸣鸣, 等. 指数施肥对杉木优良无性系生长和光合特性的影响[J]. 应用与环境生物学报, 2020, 26(2): 1-13.
[20] 叶子飘. 光合作用对光和CO₂响应模型的研究进展[J]. 植物生态学报, 2010, 34(6): 727-740.
[21] 李义博, 宋贺, 周莉, 等. C4植物玉米的光合-光响应曲线模拟研究[J]. 植物生态学报, 2017, 41(12): 1289-1300.
[22] 俞新妥, 傅瑞树. 不同种源杉木光合性状的比较研究[J]. 福建林学院学报, 1989, 9(3): 223-237.
[23] 周丽, 陈诗, 徐杨, 等. 云南松不同种源苗木光合特性比较[J]. 西南林业大学学报, 2015, 35(5): 8-13.
[24] 彭华贵, 李兆佳, 周志平, 等. 4个杉木品系在广东省天井山林场的生长比较[J]. 林业与环境科学, 2017, 33(4): 25-28.
[25] TRIEU NB.越南杉木优良种源早期选择与中国杉木种源的比较研究[D]. 福州: 福建农林大学, 2016.
[26] 吴明钦, 孙玉军, 郭孝玉, 等. 长白落叶松树冠体积和表面积模型[J]. 东北林业大学学报, 2014, 42(5): 1-5.
[27] 肖春旺, 刘玉成. 不同光环境的四川大头茶幼苗的生态适应[J]. 生态学报, 1999, 19(3): 134-138.
[28] ABER J, MCDOWELL W, NADELHOFFER K, et al. Nitrogen saturation in temperate forest ecosystems hypotheses revisited[J]. Bioscience, 1998, 48(11): 921-934.
[29] CHEN L, DONG T, DUAN B.Sex-specific carbon and nitrogen partitioning under N deposition in Populus cathayana[J]. Trees, 2014, 28(3): 793-806.
[30] 刘奇华, 周学标, 杨连群, 等. 生育前期遮光对水稻灌浆期剑叶生理特性及籽粒生长的影响[J]. 应用生态学报, 2009, 20(9): 2135-2141.
[31] 王凯, 朱教君, 于立忠, 等. 遮阴对黄波罗幼苗的光合特性及光能利用效率的影响[J]. 植物生态学报, 2009, 33(5): 1003-1012.
[32] 李延菊, 李宪利, 张序, 等. 3个扁桃品种的光合特性[J]. 林业科学, 2006, 42(11): 23-28.
[33] 王忠. 植物生理学[M]. 北京: 中国农业出版社, 2000: 107-109.
[34] SCHREEG L A, SANTIAGO L S, WRIGHT S J, et al.Stem,root,and older leaf N:P ratios are more responsive indicators of soil nutrient availability than new foliage[J]. Ecology, 2014,95(8): 2062-2068.
[35] ZHANG J, ZHAO N, LIU C, et al.C:N:P stoichiometry in China's forests:From organs to ecosystems[J]. Functional Ecology, 2018,32(1): 50-60.
[36] HAN W, FANG J, GUO D, et al.Leaf Nitrogen and phosph-orus stoichiometry across 753 terrestrial plant species in China[J]. The New Phytologist, 2005, 168(2): 377-385.
[37] GUSEWELL S, KOERSELMAN W.Variation in nitrogen and phosphorus concentrations of wetland plants[J]. Perspectives in Plant Ecology, Evolution and Systematics, 2002, 5(1): 37-61.