四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (04): 409-415.doi: 10.16036/j.issn.1000-2650.2020.04.005

• • 上一篇    下一篇

弱光对苗期大豆叶片形态结构和光合荧光特性的影响

毛诗雅, 武佳丽, 高静, 何林平, 杨雪琴, 杨峰*   

  1. 四川农业大学农学院/农业部西南作物生理生态与耕作重点实验室,成都 611130
  • 收稿日期:2020-05-25 出版日期:2020-08-28 发布日期:2021-02-02
  • 作者简介:毛诗雅,在读本科生。*责任作者:杨峰,博士,教授,主要从事研究方向为作物生理生态研究,E-mail:f.yang@sicau.edu.cn。
  • 基金资助:
    国家自然科学基金项目(31571615); 大学生科研兴趣培养计划(1911113118)

Effects of Shading on Morphological Structure and Photosynthetic Fluorescence Characteristics of Seeding Soybean Leaves

MAO Shiya, WU Jiali, GAO Jing, HE Linping, YANG Xueqin, YANG Feng*   

  1. College of Agronomy,Sichuan Agricultural University/Key Laboratory of Crop Physiology,Ecology and Cultivation in Southwest China, Ministry of Agriculture,Chengdu 611130,China
  • Received:2020-05-25 Online:2020-08-28 Published:2021-02-02

摘要: 【目的】探究弱光环境对苗期大豆叶片形态结构及光合荧光特性的影响。【方法】采用两因素完全随机设计盆栽试验,以耐荫品种南豆12和荫蔽敏感品种桂夏3号为研究对象,设置CK(正常光照)、A1(遮光度69.7%)和A2(遮光度86.3%)3个处理,分析不同弱光处理下苗期大豆叶片的形态、解剖结构、光合色素含量和光合荧光特性的响应。【结果】与CK相比,弱光下大豆叶面积增大,比叶重、栅栏组织厚度、海绵组织厚度和叶片厚度均下降,且下降幅度随遮光程度的增加而增加,南豆12叶片的栅海比先增大后减小,最大值出现在处理A1(1.36),显著高于桂夏3号。大豆叶片叶绿素a含量、净光合速率、实际量子产量在遮荫处理下均有不同程度的下降,而非光化学淬灭系数随着遮荫程度的增加呈先升高后降低趋势。对于不同品种,弱光环境下,南豆12的光合特性优于桂夏3号。在强遮荫处理A2下,南豆12叶片叶绿素含量及光合荧光参数变化幅度都小于桂夏3号,且净光合速率更高。【结论】光强直接影响到大豆叶片的形态结构及光合荧光特性,且不同耐荫性大豆品种的叶片结构和光合荧光特性对弱光环境的响应存在差异。

关键词: 大豆, 光强, 叶片结构, 光合作用, 荧光特性

Abstract: 【Objective】 Light environment plays an important role in crop growth and development, the objective of this study is to explore the effects of shading on leaf morphological structure and photosynthetic fluorescence characteristics of seeding soybean leaves. 【Method】 Nandou 12 (shade-tolerant) and Guixia 3 (shade-sensitive) were used as material. Three treatments were adapted including CK (normal light),A1 (shading rate 69.7% of CK), A2 (shading rate 86.3% of CK). Two-factor randomized block design was used for analyzing the responses of different soybean varieties to shade by the measurement of morphological structure, photosynthetic pigment content and photosynthetic fluorescence characteristics of seeding soybean leaves. 【Result】 Compared with CK, under the A1 and A2 treatment,two soybean varieties both showed that, leaf area increased, the thickness of palisade tissue, sponge tissue and leaf reduced, and the decrease amplitude increased with the increase of shade degree. Moreover, Palisade tissue/sponge tissue of Nandou 12 increased first and then decreased, with a maximum value of 1.36 under A1 treatment, which was significantly higher than Guixia 3. Shading reduced chlorophyll a content, net photosynthetic rate and actual quantum yield of both two soybean varieties in different degrees, while as the degree of shading increases, the non-photochemical quenching coefficients of soybean leaf increased first and then decreased. For two varieties, under low-light environment, the photosynthetic characteristics of Nandou 12 were better than Guixia 3. Under the strong shade treatment A2, the change amplitude of chlorophyll content per leaf area and photosynthetic fluorescence parameters of Nandou 12 leaves were all less than Guixia 3, and the net photosynthetic rate of Nandou 12 was higher. 【Conclusion】 Light directly affects the morphological structure and photosynthetic fluorescence characteristics of soybean leaves, but the leaf structure and photosynthetic fluorescence characteristics of different soybean varieties have different responses to low-light environments.

Key words: soybean, light intensity, leaf structure, photosynthesis, fluorescence characteristics

中图分类号: 

  • S311