四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (04): 373-383.doi: 10.16036/j.issn.1000-2650.2020.04.001

• •    下一篇

普通小麦TaGNOM1的分子鉴定和表达分析

李婷, 涂洋, 李聪, 刘航, 兰秀锦, 马建*   

  1. 四川农业大学小麦研究所,成都 611130
  • 收稿日期:2019-11-20 出版日期:2020-08-28 发布日期:2021-02-02
  • 作者简介:李婷,硕士研究生。*责任作者:马建,博士,教授,主要从事小麦产量性状基因的定位、分离、功能分析及其育种应用等研究,E-mail:jianma@sicau.edu.cn。
  • 基金资助:
    国家自然科学基金(31971937)

Molecular Characterization and Expression Analysis of TaGNOM1 in Common Wheat

LI Ting, TU Yang, LI Cong, LIU Hang, LAN Xiujin, MA Jian*   

  1. Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2019-11-20 Online:2020-08-28 Published:2021-02-02

摘要: 【目的】初步了解GNOM1基因在小麦中的可能的功能。【方法】对小麦同源基因TaGNOM1进行了结构、启动子调控区、组织特异性表达以及非生物胁迫表达等进行了分析,同时利用实时荧光定量PCR技术(RT-qPCR)在“中国春”中对该基因的表达模式进行了验证。【结果】TaGNOM1位于小麦第4同源染色体群(4A、4B和4D),其基因结构一致,包含1个内含子和2个外显子。在所研究的物种中除大麦外,均包含有保守的功能域(Sec7)。组织特异性表达分析表明,与其他组织相比GNOM1基因在小麦的穗、茎和根部,水稻的花药、穗及根部,以及大麦的花序轴表达更为丰富。公共数据库和RT-qPCR分析结果表明小麦TaGNOM1在高盐处理下,表达量受到正向调控;而在低温、ABA、干旱和高温处理下,受到负向调控。这些结果表明该基因可能参与小麦非生物逆境胁迫的调控。【结论】拓宽了对TaGNOM1基因的认识,对进一步研究其在小麦中的生物学功能提供了参考信息。

关键词: 普通小麦, TaGNOM1, 基因结构, 蛋白结构, 转录因子, 表达模式, 非生物胁迫

Abstract: 【Objective】 To investigate the biological function of GNOM1 gene in wheat. 【Method】 We analyzed its gene and protein structures, predicted the transcription factor binding sites of its promoter, and further obtained its expression patterns by online database. Furthermore, the expression pattern of GNOM1 gene was verified using real-time quantitative reverse transcription PCR (RT-qPCR) in Chinese Spring. 【Result】 TaGNOM1 was located on the group 4 chromosomes (4A, 4B and 4D). It was composed of two exons and one intron. A highly conserved functional domain (Sec7) was detected in all species except barley. GNOM1 gene was mainly expressed in wheat spike, stem and root;in rice anther, spike and root; and in barley rachis compared to other tissues. Public database and RT-qPCR based analyses showed that TaGNOM1 was up-regulated under high salinity treatment and down-regulated under cold, ABA, drought and heat treatments. These results further indicated that TaGNOM1 may be involved in regulation under abiotic stresses in wheat. 【Conclusion】 Taken together, this study broadens our knowledge on TaGNOM1 and will contribute to the study of its biological function in wheat and provide important information for crop genetic improvement.

Key words: gene structure, protein structure, transcription factor, expression pattern, abiotic stress, common wheat, TaGNOM1, gene structure, protein structure, transcription factor, expression pattern, abiotic stress

中图分类号: 

  • S512.1+1