酵母硒对中蜂的工蜂初生重、蜂蜜硒含量及抗氧化活性的影响

doi:10.16036/j.issn.1000-2650.2020.03.016

四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (03): 359-364.doi: 10.16036/j.issn.1000-2650.2020.03.016

酵母硒对中蜂的工蜂初生重、蜂蜜硒含量及抗氧化活性的影响

李为常¹, 何伟东¹, 潘柳初¹, 欧阳菠¹, 黄伦², 王遂林³, 王建文⁴, 王顺海⁴, 赖康⁴, 杨明显^1,*

1.四川农业大学动物科技学院,成都 611130;
2.甘孜州蜂业管理站,四川康定 626000;
3.马尔康市农业畜牧局,四川马尔康 624000;
4.四川省蜂业管理站,成都 610041

收稿日期:2019-09-26 出版日期:2020-06-28 发布日期:2021-02-03
作者简介:李为常,硕士研究生。^*责任作者：杨明显,副教授,主要从事蜜蜂生物学研究,E-mail：MX.Yang@sicau.edu.cn。
基金资助:
四川省“十三五”育种攻关项目（2016NYZ0048）

Effects of Yeast Selenium on Selenium Content and Antioxidant Activity in Honey, and on the Birth Weight of Worker Bees (Apis cerana)

LI Weichang¹, HE Weidong¹, PAN Liuchu¹, OUYANG Bo¹, HUANG Lun², WANG Suilin³, WANG Jianwen⁴, WANG Shunhai⁴, LAI Kang⁴, YANG Mingxian^1,*

1. College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;
2. Beekeeping Management Station of Ganzi State,Kangding 626000,Sichuan,China;
3. Maerkang City Bureau of Agriculture and Animal Husbandry,Maerkang 624000,Sichuan,China;
4. Beekeeping Management Station of Sichuan,Chengdu 610041,China

Received:2019-09-26 Online:2020-06-28 Published:2021-02-03

摘要/Abstract

摘要： 【目的】研究酵母硒对中蜂的工蜂初生重、蜂蜜硒含量及抗氧化活性的影响。【方法】选择12群中蜂随机分成4组。对照组仅饲喂糖水,试验组饲喂的糖水中酵母硒添加水平分别为0.2、0.4、0.6 g/kg。然后测工蜂初生重,蜂蜜的硒含量及其抗氧化活性的相关指标。【结果】试验组工蜂初生重显著高于对照组（P<0.05）,但试验组之间差异不显著（P>0.05）;蜂蜜的硒含量、清除DPPH自由基能力、蜂蜜清除羟基自由基能力、蜂蜜抗超氧阴离子自由基能力随糖水中酵母硒水平的增加而升高。【结论】糖水中酵母硒添加水平从0.2 g/kg到0.6 g/kg时能提高工蜂的初生重、蜂蜜的硒含量和抗氧化活性。

关键词: 酵母硒, 中蜂, 抗氧化能力, 蜂蜜硒含量, 工蜂初生重

Abstract: 【Objective】 The purpose of this study was to investigate the effects of yeast selenium on the birth weight of worker bees (Apis cerana), selenium content, and antioxidant activity in honey. 【Method】Twelve honeybee colonies of Apis cerana were chosen as experimental colonies which were randomly assigned into 4 groups. Control group was fed with only sugar water, the levels of yeast selenium in the sugar water of the experimental groups were 0.2, 0.4 and 0.6 g/kg respectively. Then the birth weight of worker bees, selenium content of honey and the antioxidant activity of honey were measured. 【Result】 The birth weight of worker bees in the experimental groups were significantly higher than that of the control group (P<0.05), but the differences among the experimental groups were not significant (P>0.05). The selenium content, DPPH scavenging ability, hydroxyl radical scavenging ability, and anti-superoxide anion radical capacity in honey increased with the increase of yeast selenium level in sugar water. 【Conclusion】 Adding yeast selenium from 0.2 g/kg to 0.6 g/kg in sugar water could improve the birth weight of worker bees, selenium content and antioxidant activity in honey.

Key words: yeast selenium, Apis cerana, antioxidant activity, selenium content in honey, birth weight of worker bees

中图分类号:

S896

李为常, 何伟东, 潘柳初, 欧阳菠, 黄伦, 王遂林, 王建文, 王顺海, 赖康, 杨明显. 酵母硒对中蜂的工蜂初生重、蜂蜜硒含量及抗氧化活性的影响[J]. 四川农业大学学报, 2020, 38(03): 359-364.

LI Weichang, HE Weidong, PAN Liuchu, OUYANG Bo, HUANG Lun, WANG Suilin, WANG Jianwen, WANG Shunhai, LAI Kang, YANG Mingxian. Effects of Yeast Selenium on Selenium Content and Antioxidant Activity in Honey, and on the Birth Weight of Worker Bees (Apis cerana)[J]. Journal of Sichuan Agricultural University, 2020, 38(03): 359-364.

参考文献

[1] BOSLL M R, TAKAKU K, OSHIMA M, et al.Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene[J]. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(11): 5531-5534.
[2] ROTRUCK J T, POPE A L, GANTHER H E, et al.Selenium: biochemical role as a component of glutathione peroxidase[J]. Science, 2010, 38(8): 280-283.
[3] CAI S J, WU C X, GONG L M, et al.Effects of nano-selenium on performance, meat quality, immune function, oxidation resistance, and tissue selenium content in broilers[J]. Poultry Science, 2012, 91(10): 2532-2539.
[4] ZARCZYNSKA, KATARZYNA, SOBIECH, et al. Effects of selenium on animal health[J]. Journal of Elementology, 2013, 18(2): 329-340.
[5] SALONEN J T, ALFTHAN G, HUTTUNEN J K, et al.Association between cardiovascular death and myocardial infarction and serum selenium in a matched-pair longitudinal study[J]. Lancet, 1982, 320(8291): 175-179.
[6] SALONEN J T, ALFTHAN G, HUTTUNEN J K, et al.Association between serum selenium and the risk of cancer[J]. American Journal of Epidemiology, 1984, 120(3): 342-349.
[7] WILLETT W C, POLK B F, MORRIS J S, et al.Prediagnostic serum selenium and risk of cancer[J]. Lancet, 1983, 322(8342): 130-134.
[8] VOGT T M, ZIEGLER R G, GRAUBARD B I, et al.Serum selenium and risk of prostate cancer in U.S. blacks and whites[J]. International Journal of Cancer, 2003, 103(5): 664-670.
[9] 段亮亮. 硒的生理功能和富硒保健食品开发[J]. 现代食品, 2018(1):42-45.
[10] 林长光. 硒对猪生产与保健的影响及富硒猪肉生产关键技术研究[D]. 福州: 福建农林大学,2013.
[11] 赵晓旭,杨佐君,滑静,等. 硒化壳聚糖对种公鸡组织硒含量、硒酶活性及其基因表达的影响[J]. 动物营养学报, 2013, 25(5): 1085-1092.
[12] PAYNE R L,SOUTHERN L L.Comparison of inorganic and organic selenium sources for broilers[J]. Poultry Science, 2005,84(6): 898-902.
[13] PHIPPS R H,GRANDISON A S,JONES A K,et al.Selenium supplementation of lactating dairy cows: effects on milk pro-duction and total selenium content and speciation in blood,milk and cheese[J]. Animal An International Journal of Animal Bioscience, 2008, 2(11): 1610-1618.
[14] 曾志将, 谢宪兵, 颜伟玉,等. 营养杂交对工蜂初生重的影响[J]. 经济动物学报, 2005, 9(3): 149-151.
[15] 余显伦, 赵芬萍, 彭佳华, 等. 微波消解ICP-MS测定不同产地天麻中的硒含量[J]. 西部林业科学, 2018, 47(1): 127-129.
[16] 王静丽. 微波消解ICP-MS测定蜂蜜中重金属含量[J]. 齐齐哈尔大学学报(自然科学版), 2018, 34(1): 64-66.
[17] LARRAURI J A.Effect of temperature on the free radical scavenging capacity of extracts from red and white grape pomace peels[J]. Journal of Agricultural & Food Chemistry, 1998, 46(7): 2694-2697.
[18] TAKAKO YOKOZAWA T, ERBO DONG T, TAKAKO NAKAGAWA T, et al.In vitro and in vivo studies on the radical-scavenging activity of tea[J]. Journal of Agricultural & Food Chemistry, 1998, 46(6): 2143-2150.
[19] 程艳华, 刘亚男, 胡福良,等. 蛋白质营养水平对工蜂初生重和咽下腺发育的影响[J]. 中国蜂业, 2008, 59(12): 11-13.
[20] HOGAN G R, RAZNIAK H G.Selenium-Induced mortality and tissue distribution studies in tenebrio molitor (coleoptera: tenebrionidae) oxford academic[J]. Environmental Entomology, 1991, 20(3): 790-794.
[21] 罗仕琼. 蜂蜜的体外抗氧化研究[D]. 广州: 暨南大学, 2017.
[22] 曹炜, 卢珂, 陈卫军,等. 不同种类蜂蜜抗氧化活性的研究[J]. 食品科学, 2005, 26(8): 352-356.
[23] SINGLETON V, ROSSI J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J]. American Journal of Enology and Viticulture, 1964, 16(3): 144-158.
[24] 王伟, 张玉, 朱作艺, 等. 西双版纳野生蜂蜜营养成分含量及抗氧化活性[J]. 浙江农业科学, 2017, 58(4): 691-694.
[25] BURK R F.Selenium, an antioxidant nutrient[J]. Nutrition in Clinical Care, 2010, 5(2): 75-79.

酵母硒对中蜂的工蜂初生重、蜂蜜硒含量及抗氧化活性的影响

Effects of Yeast Selenium on Selenium Content and Antioxidant Activity in Honey, and on the Birth Weight of Worker Bees (Apis cerana)

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

Metrics

本文评价

推荐阅读 10

[1]	蔡景义, 曾绘锦, 曾亭轩, 焦艳娜, 贾刚, 赵华, 田刚. 饲粮中不同比例玉米皮对生长肉兔肠道形态、消化酶活性、抗氧化能力、免疫功能及盲肠发酵参数的影响[J]. 四川农业大学学报, 2021, 39(2): 240-247.
[2]	安菁, 刘欢语, 郑艳, 程俊, 宋春. 土壤微塑料残留对大豆幼苗生长及生理生化特征的影响[J]. 四川农业大学学报, 2021, 39(1): 41-46.
[3]	李锦英, 陈代文, 郑萍, 虞洁, 毛湘冰, 何军, 黄志清, 罗玉衡, 罗钧秋, 余冰. 饲粮添加陈化玉米和复合抗氧化剂对仔猪生长性能、抗氧化功能及肝脏损伤的影响[J]. 四川农业大学学报, 2019, 37(01): 83-91.
[4]	迟晓枫丁雪梅. 饲粮中添加壳寡糖对肉鸡抗氧化性能的影响[J]. , 2017, 35(04): 568-573.
[5]	张泽宏, 朱丽霞, 胡俊西, 章家恩. 福寿螺肉提取液抗氧化活性初步研究[J]. 四川农业大学学报, 2014, 32(04): 451-454.
[6]	张纯, 温安祥. 绿原酸对建鲤生长、非特异性免疫功能和抗氧化能力的影响[J]. 四川农业大学学报, 2012, 30(01): 92-97.