四川农业大学学报 ›› 2020, Vol. 38 ›› Issue (03): 346-352.doi: 10.16036/j.issn.1000-2650.2020.03.014

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胶体金免疫层析技术在检测乳制品中三聚氰胺的应用

潘声旺1, 和垚1, 何茂萍1, 陈春坛1,2, 周钰潇1, 何靓雯1, 李亚阑1,2,*   

  1. 1.成都大学建筑与土木工程学院,成都 610106;
    2.四川文理学院化学化工学院,四川 达州 635000
  • 收稿日期:2019-10-27 出版日期:2020-06-28 发布日期:2021-02-03
  • 作者简介:潘声旺,博士,教授。*责任作者:李亚阑,硕士,副研究员,主要从事农业环境保护与食品安全研究,E-mail:364025638@qq.com。
  • 基金资助:
    成都市科技局技术创新研发计划(2018-YF05-00486-SN); 四川省教育厅科研项目(18ZA0116)

A Rapid Colloidal Gold-Based Immunochromatographic Strip Assay for Monitoring Melamine in Dairy Products

PAN Shengwang1, HE Yao1, HE Maoping1, CHEN Chuntan1,2, ZHOU Yuxiao1, HE Jingwen1, LI Yalan1,2,*   

  1. 1. School of Architecture and Civil Engineering,Chengdu University,Chengdu 610106,China;
    2. School of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou 635000,Sichuan,China
  • Received:2019-10-27 Online:2020-06-28 Published:2021-02-03

摘要: 【目的】基于竞争抑制免疫层析原理,建立一种快速检测乳制品中三聚氰胺的方法。【方法】在胶体金免疫层析(GICA)技术的基础上,以胶体金为标记物,建立乳制品中三聚氰胺(MA)快速检测方法、研发GICA检测试纸;验证该方法的灵敏度、重复性和特异性。【结果】所研发的GICA试纸测定乳制品中MA的检出限为40~160 μg/kg,重现性好;选用11种干扰物对GICA试剂进行特异性实验,仅发现尿素、缩二脲对GICA试纸检测液态奶时存在干扰,其他9种无干扰;使用GICA、HPLC、GC-MS同时检测阴性或添加MA质量分数2.00 mg/kg的阳性乳样品,检测结果一致。【结论】GICA试纸适用于乳制品中MA的快速检测。

关键词: 胶体金, 免疫层析, 三聚氰胺, 乳制品

Abstract: 【Objective】 This study was aimed to establish a method for the rapid detection of melamine(MA) in dairy products based on the principle of competitive immunochromatography. 【Method】 On the basis of colloidal gold Immunochromatographic assay (GICA) technology, a rapid detection method for MA in dairy products was established and GICA test paper was developed with colloidal gold as the marker, and its sensitivity, repeatability and specificity were verified. 【Result】 Determination of melamine in dairy products by colloidal gold immuneochromatographic assay the detection limits for 40~160 μg/kg,and reproducibility is good. Using 11 kinds of interferences of colloidal gold immuneochromatographic reagent strip for the specific experiment,discovered the presence of biuret and urea interference on colloidal gold immuneochromatographic reagent strip for detection of liquid milk,other substances without any interference. Simultaneous determination of the negative milk samples or the positive milk samples with MA content of 2.00 mg/kg by GICA and high performance liquid chromatography(HPLC), and gas chromatography-mass spectrometer (GC-MS) the results obtained by these three methods were in good agreement. 【Conclusion】 This method is suitable for rapid detection of melamine in dairy products.

Key words: colloidal gold, immunochromatography, melamine, dairy products

中图分类号: 

  • TS207.7