关键词: 副猪嗜血杆菌, 分离与鉴定, 药敏试验, 多重PCR, 血清型分型

Abstract: 【Objective】 The purpose of this study was to determine the serotypes of suspected Haemophilus parapigs in diseased sample from a pig farm in Henan Province, and to provide a drug regimen. 【Method】 The strain was isolated and cultured in the laboratory, identified by biochemistry, drug sensitivity test and serotyping. 【Result】 The results showed that Gram staining was negative short bacillus, which was polymorphic. It fermented glucose, fructose, sucrose, maltose, mannitol, xylose, urea, oxidase negative, nitrate reductase, contact enzyme positive, MR and VP test negative, and therewas satellite phenomenon;The target fragment of 821 bp was amplified by 16Sr RNA, and the homology between the sequencing results and the NR_118757.1 of Haemophilus parasuis published by GenBank was more than 98%. It was sensitive to 8 antibiotics including Ceftiofur, Amikacin, Colistin Sulfate, Thiamphenicol, Kanamycin, Gentamicin, Doxycycline, Florfenicol,and was resistant to Amoxicillin and Enrofloxacin. The serotyping of the isolated bacteria was carried out with 14 pairs of typing primers, and the target gene fragment about the size of 700 bp was amplified by multiplex PCR. The homology between the sequencing results and the funV published by GenBank of KC795429.1 was 99.85%, which was confirmed that this isolated strain was serovars 9 Hemophilus paracinis. 【Conclusion】 The results provided reference for the prevention and treatment of Haemophilus parapigs.

Key words: Haemophilus paramyclosus, isolation and identification, drug sensitivity test, multiple PCR, serotyping