四川农业大学学报 ›› 2019, Vol. 37 ›› Issue (04): 434-441.doi: 10.16036/j.issn.1000-2650.2019.04.002

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长江中下游麦区小麦地方种质千粒重全基因组关联分析

程宇坤1, 李健1, 姚方杰1, 蒋云峰1, 江千涛1, 李伟2, 蒲至恩2, 邓梅1, 魏育明1, 陈国跃1,*   

  1. 1.四川农业大学小麦研究所,成都 611130;
    2.四川农业大学农学院,成都 611130
  • 收稿日期:2019-01-24 出版日期:2019-08-28 发布日期:2021-01-31
  • 作者简介:程宇坤,博士研究生。*责任作者:陈国跃,博士,教授,主要从事小麦优异基因发掘与利用研究,E-mail:gychen@sicau.edu.cn。
  • 基金资助:
    国家重点研发计划(2016YFD0102000); 四川省科技厅国际科技创新合作项目(2019YFH0063)

Genome-Wide Association Study of Thousand Kernel Weight in Chinese Wheat Landraces in the Middle and Lower Reaches of the Yangtze River

CHENG Yukun1, LI Jian1, YAO Fangjie1, JIANG Yunfeng1, JIANG Qiantao1, LI Wei2, PU Zhien2, DENG Mei1, WEI Yuming1, CHEN Guoyue1,*   

  1. 1. Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;
    2. College of Agronomy,Sichuan Agricultural University,Chengdu 611130,Sichuan,China
  • Received:2019-01-24 Online:2019-08-28 Published:2021-01-31

摘要: 【目的】高产是小麦育种的永恒主题,千粒重是产量构成的三因子之一。因此,发掘控制小麦千粒重QTL(quantitative trait loci)区段及其优异等位变异,对利用分子标记辅助选择进行小麦产量性状的遗传改良具有重要意义。【方法】以来自长江中下游麦区188份小麦地方种质为材料,在3个环境下对小麦千粒重进行表型鉴定;利用DArT芯片(diversity arrays technology)进行全基因组扫描,基于一般线性模型(Q+K)对小麦千粒重进行关联分析。【结果】千粒重表型鉴定发现来自江苏、浙江和湖北小麦地方种质具有较高千粒重;通过全基因关联分析,共获得8个DArT标记在2个及以上的环境中均与小麦千粒重显著关联,分布于小麦染色体1B、1D、3B、4B和5B上,其表型贡献率9.19%~12.65%。【结论】长江中下游麦区小麦地方种质千粒重表型变异丰富;在不同环境中均能关联到的标记为较为稳定的控制小麦千粒重位点。

关键词: 千粒重, 关联分析, 小麦地方种质, DArT芯片

Abstract: 【Objective】 High yield is the eternal theme of wheat breeding program and thousand kernel weight is one of the three factors of yield components. For exploring wheat grain weight QTLs and its allelic variations are great significance on genetic improvement of wheat yield traits using Marker-assisted selection. 【Method】 By investigated thousand kernel weight traits using 188 Chinese wheat lan-draces from the middle and lower reaches of the Yangtze River across three environments. Genome-wide association analysis was performed using wheat DArT-seq (diversity arrays technology) with general linear Q+K association models. 【Result】 The accessions, derived from Jiangsu, Zhejiang and Hubei Province,had good performance for thousand kernel weight traits. A total of 8 significant marker-trait associations (MTAs) were detected on chromosomes 1B, 1D, 3B, 4B, and 5B in more two environments. Single locus could explain 9.19% to 12.65% the observed phenotypic variation. 【Conclusion】 The thousand kernel weight traits performance are significant difference in Chinese wheat landraces from the middle and lower reaches of the Yangtze River; The stable loci associated with the thousand kernel weight are found in different environments.

Key words: thousand kernel weight, genome-wide association analysis, wheat landraces, DArT-seq

中图分类号: 

  • S326