低出生重对仔猪肝脏抗氧化功能的影响及精氨酸的营养效应

doi:10.16036/j.issn.1000-2650.2019.03.015

摘要/Abstract

摘要： 【目的】旨在探讨低出生重（LBW）对仔猪肝脏抗氧化功能的影响及饲粮添加精氨酸的营养效应。【方法】试验1选取10头正常出生重（NBW）仔猪和10头LBW仔猪,出生后2~4 h内屠宰,比较出生体重对新生仔猪肝脏抗氧化能力的影响。试验2选取4日龄的10头NBW和20头LBW仔猪,NBW仔猪饲喂基础饲粮,LBW仔猪分为两组,分别饲喂基础饲粮和添加1.0% L-精氨酸的饲粮,均配制成人工乳,饲喂21 d,考察出生体重对哺乳仔猪肝脏抗氧化功能的影响及精氨酸的营养效应。【结果】与NBW新生仔猪相比,LBW新生仔猪肝脏T-SOD和GSH-Px活性显著降低（P<0.05）。饲喂21 d后,与NBW仔猪相比,LBW仔猪肝脏ASA和AHR活性显著降低,Nrf2和NQO-1表达量显著提高（P<0.05）。与LBW仔猪相比,1.0% Arg添加显著提高了LBW仔猪肝脏AHR、T-SOD和GSH-Px的活性,显著降低NQO-1、IL-1β和IL-6的表达量（P<0.05）。【结论】在本试验条件下,LBW仔猪肝脏抗氧化能力较NBW仔猪低,补充1.0% Arg可改善LBW哺乳仔猪肝脏抗氧化功能。

关键词: 低出生重仔猪, 精氨酸, 抗氧化, 细胞因子

Abstract: 【Objective】 The aim of this study was to investigate the effects of low birth weight (LBW) on antioxidant function in liver, and the nutritional effects of arginine supplementation on piglets. 【Method】 In exp.1,ten normal birth weight (NBW) and ten low birth weight (LBW) piglets were selected and slaughtered within 2-4 hours after birth to study the effects of birth weight on the antioxidant function in liver of neonatal piglets. In exp. 2, a total of thirty suckling piglets(4 days old) including ten NBW piglets and twenty LBW piglets were selected. NBW piglets were fed with basal diet, and LBW piglets were fed with basal diet or diet supplemented with 1.0% L-arginine,made into artificial milk. The experiment lasted 21 days. 【Result】 Compared with NBW neonatal piglet, LBW neo-natal piglets significantly decreased the activity of T-SOD and GSH-Px in liver(P<0.05). Compared with NBW suckling piglets,LBW suckling piglets significantly decreased the activity of ASA and AHR,significantly increased the mRNA expressions of Nrf 2 and NQO-1 in liver(P<0.05). Compared with LBW suckling piglets were fed with basal diets, LBW suckling piglets supplementation with 1.0% L-arginine significantly increased the activity of AHR, T-SOD and GSH-Px, significantly decreased the mRNA expressions of NQO-1,IL-1β and IL-6 in liver (P<0.05). 【Conclusion】 The LBW piglets exhibit lower antioxidant function in liver, and dietary supplementation 1.0% L-Arg improves the antioxidant function in liver of LBW piglets.

Key words: low birth weight piglet, arginine, antioxidant, cytokine

中图分类号:

S828

田一航, 宋毅, 陈代文, 余冰, 何军, 虞洁, 毛湘冰, 罗玉衡, 黄志清, 罗钧秋, 郑萍. 低出生重对仔猪肝脏抗氧化功能的影响及精氨酸的营养效应[J]. 四川农业大学学报, 2019, 37(03): 383-389.

TIAN Yihang, SONG Yi, CHEN Daiwen, YU Bing, HE Jun, YU Jie, MAO Xiangbing, LUO Yuheng, HUANG Zhiqing, LUO Junqiu, ZHENG Ping. Effects of Low Birth Weight on the Antioxidant Function in Liver of Piglets and the Nutritional Regulation of L-Arginine[J]. Journal of Sichuan Agricultural University, 2019, 37(03): 383-389.

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