四川农业大学学报 ›› 2019, Vol. 37 ›› Issue (02): 276-282.doi: 10.16036/j.issn.1000-2650.2019.02.020

• • 上一篇    

香芹酚对耐甲氧西林金黄色葡萄球菌(USA300)抑菌机制的研究

甘盈盈, 袁中伟, 张天翼, 谷可欣, 李超, 申瀚君, 周能华, 尹立子*   

  1. 四川农业大学动物医学院,成都 611130
  • 收稿日期:2018-12-21 出版日期:2019-04-28 发布日期:2021-01-29
  • 作者简介:甘盈盈,本科生。 *责任作者:尹立子,博士,副教授,主要从事中药抗细菌毒力因子及其应用的研究,E-mail:yinlizi@sicau.edu.cn。
  • 基金资助:
    国家自然科学基金(31702284)

Antibacterial Mechanism of Carvacrol Against Methicillin-Resistant Staphylococcus aureus(USA300)

GAN Yingying, YUAN Zhongwei, ZAHNG Tianyi, GU Kexin, LI Chao, SHEN Hanjun, ZHOU Nenghua, YIN Lizi*   

  1. College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2018-12-21 Online:2019-04-28 Published:2021-01-29

摘要: 【目的】研究香芹酚对耐甲氧西林金黄色葡萄球菌(MRSA)(USA300)的抑菌效果及其作用机制。【方法】测定香芹酚对MRSA标准菌株USA300的抑菌活性,并通过考察其对电导率、DNA外渗量、SDS-PAGE蛋白谱带、乳酸脱氢酶的含量、细菌形态结构以及生物被膜形成能力的影响探究香芹酚的抑菌机制。【结果】香芹酚的MIC为128~256 μg/mL,MBC为512 μg/mL;与对照组相比,2 MIC香芹酚作用于USA300 1 h后,菌悬液电导率上升5.57%±0.40%、DNA外渗量增加16.27±1.86 μg/mL以及乳酸脱氢酶含量增长40.49%±2.77%;作用10 h后,可溶性蛋白质含量增多61.13%±7.63%;512 μg/mL的香芹酚可以损伤USA300的细胞壁;亚抑菌浓度下,64 μg/mL香芹酚作用于USA300后,形成的生物被膜减少68.86%±0.53%。【结论】香芹酚主要通过损伤USA300的细胞壁、增加细胞膜的通透性来抑制USA300的生长繁殖。

关键词: 耐甲氧西林金黄色葡萄球菌, 香芹酚, 抑菌机制

Abstract: 【Objective】 The aim of this study was to investigate the antibacterial effect and the mechanism of carvacrol against Methicillin-resistant Staphylococcus aureus (USA300). 【Method】 The antibacterial activity of carvacrol on the MRSA standard strain USA300 was determined, and the antibacterial mechanism of carvacrol was determined by exploring the influence of carvacrol on the conductivity, DNA exosmosis, SDS-PAGE protein band, LDH content, bacterial morphological structure and biofilm formation ability. 【Result】 MIC of carvacrol to USA300 was 128~256 μg/mL and MBC of carvacrol to USA300 was 512 μg/mL; Compared with the control group, after treating USA300 with 2 MIC carvacrol for 1 h, bacteria suspension conductivity was increased by 5.57%±0.40%, DNA exosmosis amount improved by 16.27±1.86 μg/mL and LDH content increased by 40.49%±2.77%; After treating for 10 h, soluble protein content was increased by 61.13%±7.63%; 2 MIC carvacrol could destroy the cytoderm of bacteria; Compared with the control group, the total amount of biofilm formed was decreased by 68.86%±0.53% after treating USA300 with 64 μg/mL carvacrol at Sub-MIC. 【Conclusion】 The antibacterial mechanism of carvacrol is mainly by destroying cytoderm and increasing cell membrane permeability of USA300.

Key words: Methicillin-resistant Staphylococcus aureus, carvacrol, antibacterial mechanism

中图分类号: 

  • S511