不同类型猪舍内气溶胶中致病菌数量和内毒素水平的比较研究

doi:10.16036/j.issn.1000-2650.2019.02.019

四川农业大学学报 ›› 2019, Vol. 37 ›› Issue (02): 271-275.doi: 10.16036/j.issn.1000-2650.2019.02.019

不同类型猪舍内气溶胶中致病菌数量和内毒素水平的比较研究

许铭洙¹, 张勇¹, 郭振东², 严鸿林^1,3,*, 张宏福³, 刘静波^1,3

1.西南科技大学生命科学与工程学院,四川绵阳 621010;
2.军事科学院军事医学研究院军事兽医研究所,长春 130122;
3.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室,北京 100193

收稿日期:2018-10-09 出版日期:2019-04-28 发布日期:2021-01-29
作者简介:许铭洙,本科生。^*责任作者：严鸿林,博士,讲师,主要从事营养与微生态、畜禽舍气溶胶形成及致病机理研究,E-mail：honglinyan@swust.edu.cn。
基金资助:
国家重点研发计划项目（2016YFD0500505）

A Comparison Study of Piggery Types on the Abundances of Pathogens and Endotoxin in Aerosol

XU Mingzhu¹, ZHANG Yong¹, GUO Zhendong², YAN Honglin^1,3,*, ZHANG Hongfu³, LIU Jingbo^1,3

1. School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,Sichuan,China;
2. Institute of Military Veterinary, Academy of Military Medical Sciences,Changchun 130122,China;
3. State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China

Received:2018-10-09 Online:2019-04-28 Published:2021-01-29

摘要/Abstract

摘要： 【目的】比较猪舍类型对舍内气溶胶致病菌数量和内毒素水平的影响。【方法】选用32栋全封闭式猪舍,共计4种类型（保育舍、育肥舍、妊娠舍和分娩舍）,每种类型猪舍8栋。采用AGI-30采样器和安德森6级采样器收集空气样品,用平板培养和RT-qPCR的方法测定需氧菌和致病菌的丰度,并测定气溶胶中内毒素的含量。【结果】保育舍需氧菌数量显著高于其他猪舍,而分娩舍需氧菌数量显著低于其他猪舍（P<0.05）。保育舍气溶胶中弯曲杆菌和大肠埃希菌的数量显著高于其他猪舍（P<0.05）。此外,保育舍气溶胶中内毒素含量显著高于其他猪舍（P<0.05）。【结论】不同猪舍类型气溶胶中致病菌数量和内毒素含量存在差异。

关键词: 猪舍类型, 气溶胶, 致病菌, 内毒素

Abstract: 【Objective】 The present study was designed to compare the abundances of pathogens and endotoxin between different piggeries types. 【Method】 In summer, a total of 32 swine buildings, which were sorted into 4 categories including nursery piggery, finishing piggery, gestation piggery and farrowing piggery (n=8), were chosen to collect aerosol samples using AGI-30 impingers and six-stage Andersen microbial samplers. The abundances of aerobic bacteria and pathogens were determined using culture-dependent method or RT-qPCR. In addition, the concentration of endotoxin in aerosol samples was also monitored. 【Result】 The colonies number of aerobic bacteria was higher in aerosols of nursery building than that in aerosols of other swine buildings (P<0.05), and the total number of aerobic bacteria was lower in aerosols of farrowing building than that in aerosols of other swine buildings (P<0.05). The abundances of aerosol Campylobacter and E. coli in nursery piggery was higher compared with other piggeries types (P<0.05). Additionally, the nursery barns exhibited higher concentration of endotoxin than other barns (P<0.05). 【Conclusion】 It can be concluded that piggery categories significantly influence the abundances of aerosol pathogens and endotoxin.

Key words: piggery types, aerosol, pathogens, endotoxin

中图分类号:

S811.5

许铭洙, 张勇, 郭振东, 严鸿林, 张宏福, 刘静波. 不同类型猪舍内气溶胶中致病菌数量和内毒素水平的比较研究[J]. 四川农业大学学报, 2019, 37(02): 271-275.

XU Mingzhu, ZHANG Yong, GUO Zhendong, YAN Honglin, ZHANG Hongfu, LIU Jingbo. A Comparison Study of Piggery Types on the Abundances of Pathogens and Endotoxin in Aerosol[J]. Journal of Sichuan Agricultural University, 2019, 37(02): 271-275.

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不同类型猪舍内气溶胶中致病菌数量和内毒素水平的比较研究

A Comparison Study of Piggery Types on the Abundances of Pathogens and Endotoxin in Aerosol

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