四川农业大学学报 ›› 2019, Vol. 37 ›› Issue (01): 8-14.doi: 10.16036/j.issn.1000-2650.2019.01.002

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苦荞转录因子基因FtMYC的克隆及其表达与花青素积累的相关性分析

姚攀锋1, 吕兵兵1, 李琪1, 董玘鑫1, 王安虎2, 吴琦1,*   

  1. 1.四川农业大学生命科学学院,四川 雅安 625014;
    2.西昌学院,四川 西昌 615000
  • 收稿日期:2018-04-28 出版日期:2019-02-28 发布日期:2021-01-29
  • 作者简介:姚攀锋,博士研究生。*责任作者:吴琦,博士,教授,主要从事植物次生代谢研究,E-mail:wuqi@sicau.edu.cn。
  • 基金资助:
    四川省科技厅国际合作项目(2015HH0047)

Cloning of Transcription Factor Gene FtMYC and Analyzing the Correlation between Its Expression and Anthocyanin Accumulation

YAO Panfeng1, LYU Bingbing1, LI Qi1, DONG Qixin1, WANG Anhu2, WU Qi1,*   

  1. 1. College of Life Science,Sichuan Agricultural University,Ya'an 625014,Sichuan,China;
    2. Xichang College,Xichang 615000,Sichuan,China
  • Received:2018-04-28 Online:2019-02-28 Published:2021-01-29

摘要: 【目的】克隆苦荞FtMYC基因,分析非生物胁迫对FtMYC基因表达及花青素含量的影响。【方法】根据苦荞转录组数据克隆FtMYC基因,对其进行生物信息学分析;通过4 ℃和UV-B胁迫处理芽期苦荞,分析胁迫后苦荞胚轴和子叶中FtMYC基因表达量与花青素积累之间的相关性。【结果】苦荞FtMYC基因DNA全长1 309 bp,由1个外显子和1个内含子构成,符合GT-AG剪切原则;cDNA序列包括1个1 287 bp的开放阅读框,编码428个氨基酸;在进化树上它与参与花青素调控的其他MYC蛋白聚为一簇;UV-B逆境胁迫处理下,苦荞胚轴中FtMYC基因表达量与花青素含量变化显著性相关(r=0.798,P<0.05),在子叶中则极显著相关(r=0.887,P<0.01);在4 ℃冷胁迫处理下苦荞胚轴中FtMYC的表达量与花青素积累没有明显的相关性(r=0.744),但在子叶中FtMYC的基因表达与花青素积累显著性相关(r=0.814,P<0.05)。此外,对FtMYC基因启动子的序列分析结果显示,pFTMYC序列包含TATA-Box和CAAT-Box等启动子核心元件以及与光、低温和胁迫应答等相关的功能元件。【结论】FtMYC基因可通过参与花青素代谢调控来参与苦荞对UV-B和寒冷等非生物胁迫的应答和适应。

关键词: 苦荞, MYC转录因子, 基因克隆, 非生物胁迫

Abstract: 【Objective】 Cloning the FtMYC gene and analyzing the effect of abiotic stress on FtMYC expression and anthocyanin content in the tartary buckwheat. 【Method】 The FtMYC gene was cloned from the data of tartary buckwheat transcriptional group,and analyzed by bioinformatic method; Tartary buck-wheat was treated with 4 ℃ and UV-B treatment,and the correlation between FtMYC expression and anth-ocyanin content was analyzed in the hypocotyl and cotyledons. 【Result】 The DNA sequence of FtMYC was 1 309 bp in length and contained 1 exon and 1 intron, and in line with the principle of GT-AG splicing principle. The cDNA of FtMYC contains a 1 287 bp ORF, which encoded 428 amino acids residues. FtMYC was clustered with other MYC proteins involved in anthocyanin regulation. Under UV-B stress, FtMYC expression was significantly correlated with the anthocyanin content in hypocotyl (r=0.798,P<0.05) and cotyledon (r=0.887,P<0.01). There was no significant correlation between the expression of FtMYC and anthocyanin accumulation in tartary buckwheat under 4 ℃ stress (r=0.744), but the expression of FtMYC in the cotyledon was significant correlation with anthocyanin accumulation (r=0.814,P<0.05). In addition,sequence analysis of the FtMYC gene promoter revealed that pFtMYC contains promoter core elements such as TATA-Box and CAAT-Box as well as functional elements related to light,hypothermia and stress responses. 【Conclusion】 The FtMYC gene could be involved in the response and adaptation of buckwheat to abiotic stresses such as UV-B and cold by regulating anthocyanin metabolism.

Key words: tartary buckwheat, MYC transcription factor, gene cloning, abiotic stress

中图分类号: 

  • Q785