云南地方绵羊品种遗传多样性分子评价

doi:10.16036/j.issn.1000-2650.2018.06.017

四川农业大学学报 ›› 2018, Vol. 36 ›› Issue (06): 822-830.doi: 10.16036/j.issn.1000-2650.2018.06.017

云南地方绵羊品种遗传多样性分子评价

梁家充¹, 朱兰¹, 孙利民², 杨红远¹, 袁跃云², 李东江¹, 洪琼花^{1, *}

1. 云南省畜牧兽医科学院/云南省畜禽遗传资源保护和种质创新工程实验室,昆明 650224;
2. 云南省家畜改良工作站,昆明 650223

收稿日期:2017-12-05 出版日期:2018-12-28 发布日期:2021-03-01
通讯作者: 洪琼花, 研究员, 硕士生导师, 从事动物遗传育种与繁殖工作, E-mail:yxh7168@126.com。
作者简介:梁家充,硕士,助理研究员,E-mail:157347112@qq.com。
基金资助:
云南省高新技术产业发展项目计划；云南省主要家畜遗传资源保存和选育关键技术研究与应用（云高新产业发展201404）。

Molecular Evaluation of Genetic Diversity of Native Sheep Breeds in Yunnan Province

LIANG Jiachong¹, ZHU Lan¹, SUN Limin², YANG Hongyuan¹, YUAN Yueyun², LI Dongjiang¹, HONG Qionghua^{1, *}

1. Yunnan Animal Science and Veterinary Institute/Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement,Kunming 650224,China;
2. Domestic Animal Breeding and Crossbreed-Improvement Station of Yunnan Province,Kunming 650225,China

Received:2017-12-05 Online:2018-12-28 Published:2021-03-01

摘要/Abstract

摘要： 【目的】利用微卫星标记分析评价8个云南地方绵羊品种的遗传多样性,为资源保护和种质创新提供参考。【方法】提取迪庆绵羊、兰坪乌骨绵羊、宁蒗黑绵羊、石屏青绵羊、腾冲绵羊、昭通绵羊、丽江绵羊和云南半细毛羊这8个云南地方绵羊品种共465只绵羊的血样总DNA,利用10个微卫星标记对这些样本进行遗传多样性,UPGMA 系统聚类和群体遗传结构分析。【结果】通过对8个绵羊群体的10个微卫星位点的分析,共发现469个基因型,160个等位基因,平均每个位点有16个等位基因。其中,在位点DYMS1、MAF214和OarFCB 304检测到的等位基因数最多,都为23个;在SRCRP5位点检测到的等位基因数最少,为7个。UPGMA 系统聚类图表明,丽江绵羊和宁蒗黑绵羊的遗传距离最近;石屏青绵羊和云南半细毛羊的遗传距离最远。群体遗传结构分析结果显示,465个样本分别来自于8个种群,这与本研究中的绵羊群体来源于8个地方的群体相符。【结论】微卫星位点SRCRSP1、OarFCB304、OarJMP58、MAF33、DYMS1、ILSTS11、MAF214、MCM140和ILSTS28可以作为有效的遗传标记用于绵羊品种的遗传多样性和遗传关系研究。除石屏青绵羊变异最小,其余7个品种都具有较为丰富的遗传多样性,它们之间的遗传分化关系与品种的地域、起源、驯化和相互引种有关。

关键词: 绵羊, 微卫星, 遗传多样性

Abstract: 【Objective】 The genetic diversity of 8 Yunnan local sheep breeds was evaluated by microsatellite markers in order to provide reference for resource protection and germplasm innovation.【Method】 The genetic diversity, UPGMA system cluster and population genetic structure analysis were applied to the total DNA extracts of 465 sheep from eight Yunnan sheep breeds including Diqing sheep, Lanping black bone sheep, Ninglang black sheep, Tengchong sheep, Zhaotong sheep, Lijiang sheep and Yunnan semi-fine wool sheep by analyzing of 10 microsatellite markers. 【Result】 The results showed that 469 genotype and 160 alleles were found in the analysis of 10 microsatellite loci, and each locus had 16 alleles. The maximum number of alleles was 23, which were found in DYMS1, MAF214 and OarFCB304, On the contrary, only 7 alleles were detected from DYMS1, MAF214 and OarFCB304. UPGMA system analysis results revealed that the genetic distance between Lijiang sheep and Ninglang black sheep was the closest, whereas the furthest genetic distance was found between blue sheep and Shiping Yunnan semi fine wool sheep. The results of population genetic structure analysis indicated that 465 samples were from 8 populations, coincided with the fact that the population of the sheep populations were from 8 places in this study. 【Conclusion】 These microsatellite loci SRCRSP1,OarFCB304, OarJMP58, MAF33, DYMS1, ILSTS11, MAF214, MCM140 and ILSTS28 can be used as effective markers for studying the genetic diversity of sheep breeds and genetic relationship. Except Shiping indigo sheep, the other seven breeds have abundant genetic diversity. The genetic differentiation among them is related to the region, origin, domestication and mutual introduction of the varieties.

Key words: sheep, SSR, genetic diversity

中图分类号:

S826.2

梁家充, 朱兰, 孙利民, 杨红远, 袁跃云, 李东江, 洪琼花. 云南地方绵羊品种遗传多样性分子评价[J]. 四川农业大学学报, 2018, 36(06): 822-830.

LIANG Jiachong, ZHU Lan, SUN Limin, YANG Hongyuan, YUAN Yueyun, LI Dongjiang, HONG Qionghua. Molecular Evaluation of Genetic Diversity of Native Sheep Breeds in Yunnan Province[J]. Journal of Sichuan Agricultural University, 2018, 36(06): 822-830.

参考文献 24

[1]	PETERS J, DRIESCH A V, HELMER D, et al.The upper Euphrates-Tigris basin: cradle of agro-pastoralism[M]. The First Steps of Animal Domestication 2004.
[2]	TAPIO M, OZEROV M, TAPIO I, et al.Microsatellite-based genetic diversity and population structure of domestic sheep in northern Eurasia[J]. Bmc Genetics, 2010, 11(1): 76.
[3]	ORGANIZATION A.Molecular genetic characterization of animal genetic resources[M]. Rome: FAO, 2012.
[4]	赵有璋. 中国养羊学[M]. 北京: 中国农业出版社, 2011.
[5]	孙庆华, 陈小兵. 微卫星标记及其在动物遗传育种中的应用[J]. 养殖技术顾问, 2013, 4(5): 49.
[6]	吕慎金, 马月辉. 微卫星标记对绵山羊品种资源的评价[J]. 黑龙江畜牧兽医, 2002(10): 11-12.
[7]	辛晓玲, 徐照学, 闫祥洲,等. 微卫星标记在家畜遗传育种上的应用[J]. 河南农业科学, 2003(7): 63-64.
[8]	TAUTZ D.Hypervariability of simple sequences as a general sour-ce for polymorphic DNA markers[J]. Nucleic Acids Research, 1989, 17(16): 6463-6471.
[9]	DODGSON J B, CHENG H H, OKIMOTO R.DNA marker technology: a revolution in animal genetics[J]. Poultry Science, 1997, 76(8): 1108.
[10]	孙业良, 谢庄, 刘守仁, 等. 微卫星标记及其在绵羊遗传和育种研究中的应用[J]. 畜牧与兽医, 2005, 37(11): 50-53.
[11]	LI X, LI K, FAN B, et al.The genetic diversity of seven pig breeds in China, estimated by means of microsatellites[J]. Asian Australasian Journal of Animal Sciences, 2000, 13(9): 1193-1195.
[12]	BAUMUNG R, CUBRICCURIK V, SCHWEND K, et al.Genetic characterisation and breed assignment in Austrian sheep breeds using microsatellite marker information[J]. Journal of Animal Breeding & Genetics,2006,123(4):265-271.
[13]	YANG C, ZHU X, SUN X.Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella)[J]. Journal of Genetics and Genomics, 2008, 35(4): 201-206.
[14]	SOLLERO B P, PAIVA S R, FARIA D A, et al.Genetic diversity of Brazilian pig breeds evidenced by microsatellite markers[J]. Livestock Science, 2009, 123(1): 8-15.
[15]	ARORA R, BHATIA S, JAIN A.Morphological and genetic characterization of Ganjam sheep[J]. Animal Genetic Resources/ressources Génétiques Animales/recursos Genéticos Animales,2010, 46(1): 1-9.
[16]	KUGONZA D R, JIANLIN H, NABASIRYE M, et al.Genetic diversity and differentiation of Ankole cattle populations in Uganda inferred from microsatellite data[J]. Livestock Science,2011, 135(2): 140-147.
[17]	DIXIT S P, VERMA N K, AGGARWAL R A K, et al. Genetic diversity and relationship among Indian goat breeds based on microsatellite markers[J]. Small Ruminant Research, 2012, 105(1-3): 38-45.
[18]	SOUZA C A, PAIVA S R, MCMANUS C M, et al.Genetic diversity and,assessment of 23 microsatellite markers for parentage testing of Santa Ines, hair sheep in Brazil[J]. Genetics & Molecular Research Gmr, 2012, 11(2): 1217-1229.
[19]	Botstein D, White R L, Skolnick M, et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 1980, 32(3):314-331.
[20]	王吉振, 储明星, 王爱国, 等. 用4个微卫星标记分析7个绵羊群体之间的遗传关系[J]. 遗传, 2004, 26(5): 637-643.
[21]	常爽, 刘丑生, 林峰, 等. 9个绵羊品种的微卫星DNA多态性分析[J]. 中国农学通报, 2010, 26(11): 13-17.
[22]	汤存伟, 余雄, 刘武军, 等. 新疆13个绵羊群体遗传多样性及遗传分化的研究[J]. 家畜生态学报, 2011, 32(1): 13-19.
[23]	柴文琼, 成述儒, 靳建华, 等. 5个绵羊群体微卫星多态性分析[J]. 扬州大学学报(农业与生命科学版), 2012, 33(1): 38-42.
[24]	吕慎金, 马月辉, 杨燕, 等. 中国西部七个地方绵羊群体微卫星DNA的遗传多样性研究[J]. 家畜生态学报, 2006, 27(4): 18-22.

云南地方绵羊品种遗传多样性分子评价

Molecular Evaluation of Genetic Diversity of Native Sheep Breeds in Yunnan Province

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