UCP1基因多态性与牛耐寒性的关联分析

doi:10.16036/j.issn.1000-2650.2018.06.015

四川农业大学学报 ›› 2018, Vol. 36 ›› Issue (06): 808-814.doi: 10.16036/j.issn.1000-2650.2018.06.015

UCP1基因多态性与牛耐寒性的关联分析

罗刚, 王杰, 赖添赋, 甘明川, 赖松家^*

四川农业大学动物遗传育种研究所,四川成都 611130

收稿日期:2018-06-19 出版日期:2018-12-28 发布日期:2021-03-01
通讯作者: 赖松家, 博士, 教授, 主要从事家畜遗传育种研究, E-mail：laisj5794@gmail.com。
作者简介:罗刚,硕士研究生。
基金资助:
中国农业研究体系（CARS-44-A-2）；四川省十三五育种研究项目（2016NYZ0046）。

Association Analysis of UCP1 Gene Polymorphism with Cold Resistance of Cattles

LUO Gang, WANG Jie, LAI Tianfu, GAN Mingchuan, LAI Songjia^*

Institute of animal genetics and breeding,Sichuan Agricultural University,Chengdu 611130,China

Received:2018-06-19 Online:2018-12-28 Published:2021-03-01

摘要/Abstract

摘要： 【目的】探究UCP1基因的多态性与牛耐寒性之间的关联性。【方法】采集3个品种,共计180头牛的耳组织样品,利用PCR扩增和产物直接测序的方法,获得牛UCP1基因6个外显子的核苷酸序列,采用生物分析软件探讨了其遗传多态性与牛耐寒性的关联性。【结果】牛UCP1基因编码区存在8个变异位点,其中有5个错义位点,相关分析结果表明,5个错义位点多态性与牛抗寒性存在显著相关性。443位点C等位基因频率和6414位点的G等位基因频率随牛群体生活海拔高度的降低而降低;牦牛在3593位点和4873有生存优势的基因型分别为AG和AA型;4973位点CC基因型是高原牛生存的必要基因型。【结论】UCP1可能是牛抗寒性状基因之一。

关键词: UCP1, 西藏牦牛, 西藏黄牛, 通江黄牛, 抗寒性, 关联分析

Abstract: 【Objective】 The aim of this study was to explore the correlation between polymorphism of bovine UCP1 gene and cold tolerance in cattles. 【Method】 The ear tissue samples of 180 cattles were collected from three cattle breeds. The nucleotide sequences of 6 exons of bovine UCP1 gene were obtained by PCR amplification and analyzed by sequencing technology. The correlation between the genetic polymorphism and the cold tolerance of cattle was studied by using bioanalysis software. 【Result】 There were 8 mutation sites in the coding region of bovine UCP1 gene, including 5 missense sites. The results of correlation analysis showed that there were significant correlations between the 5 polymorphisms of missense loci and cold resistance in cattles. The C allele frequency at 443 loci and G allele frequency at 6414 loci decreased with the decrease of the habitat altitude n. The genotype of yak at 3593 locus and 4873 was AG and AA respectively, and 4973 locus CC genotype was the necessary genotype for the survival of plateau cattles. 【Conclusion】 It could be speculated that bovine UCP1 gene may be one of the candidate genes related to cold tolerance of cattles.

Key words: UCP1, Tibetan yak, Tibetan Cattle, Tongjiang Cattle, cold resistance, association analysis

中图分类号:

Q953

罗刚, 王杰, 赖添赋, 甘明川, 赖松家. UCP1基因多态性与牛耐寒性的关联分析[J]. 四川农业大学学报, 2018, 36(06): 808-814.

LUO Gang, WANG Jie, LAI Tianfu, GAN Mingchuan, LAI Songjia. Association Analysis of UCP1 Gene Polymorphism with Cold Resistance of Cattles[J]. Journal of Sichuan Agricultural University, 2018, 36(06): 808-814.

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UCP1基因多态性与牛耐寒性的关联分析

Association Analysis of UCP1 Gene Polymorphism with Cold Resistance of Cattles

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