关键词: 家兔, PIK3CA基因, AKT3基因, SNP, 生长性状

Abstract: [Objective] This study was to investigate the polymorphisms of PIK3CA and AKT3 Genes and their association with growth traits of rabbits. [Methods] We use PCR direct sequencing to detect the polymorphisms of PIK3CA and AKT3 genes and PCR-RFLP was applied to the genotyping analysis of 711 individuals including 3 meat rabbit breeds. [Results] A total of six SNPs of PIK3CA and two SNPs of AKT3 were finally found on the genomic sequences. And there were two synonymous mutations of their coding region, one was in the exon20 of PIK3CA(c.3068 A>G) and one was in exon10 of AKT3(c.1213 A>C), respectively. As for c.3068 A>G of PIK3CA, GG genotype individuals had the lowest weight at all growth stages in Fujian black rabbits and European white rabbits. The c.1213 A>C of AKT3 only exists in European white rabbits, and AC and AA genotype were significantly associated with higher body weight than CC genotype at every growth stage(P<0.05). The combined genotypes GGCC of c.3068 A>G and c.1213 A>C was associated with significantly lower body weight compared with other five combined genotypes in European white rabbits. We also found the breed-specific polymorphism, such as AA genotype individuals were not found in Ira rabbits at PIK3CA c.3068 A>G. [Conclusion] Our results indicated that the polymorphism of both PIK3CA and AKT3 genes were significantly associated with production traits in rabbits, which would be employed in the breeding program of marker assisted selection.

Key words: rabbits, PIK3CA, AKT3, SNP, growth performance