关键词: 鹅, AKR1D1基因, 编码区, 荧光定量PCR

Abstract: [Objective] The study was to clone the AKR1D1 gene's cDNA coding domain sequence of geese and predict its structure, function and expression profiles. [Methods] Using Tianfu meat-type geese as materials, we cloned the goose's AKR1D1 coding domain sequence(CDS) by RTPCR, and predicted its protein structure and functions through several bioinformational tools and analyzed its expression in ovarian follicles of different stages of goose by real-time quantitative PCR. [Results] The results showed that the goose's AKR1D1 CDS consisted of 981 nucleotides that encoded 326 amino acids residues, and the amino acid homology of goose AKR1D1 shared 95.71% identity with chicken. The analysis of amino acid sequence revealed that the goose AKR1D1 encoded water-soluble protein and its relative molecular weight was 37 263.7 Da. Subcellular location of AKR1D1 was primarily in the cytoplasm and chondriosome, and it did not belong to the secreted protein. It is predicted that the AKR1D1 protein contained 18 phosphorylation sites, 3 glycosylation sites. The secondary structure of AKR1D1 was mainly composed of random coil, while the tertiary structure of domain area showed a formic form helix structure. The results of real-time quantitative PCR revealed that goose AKR1D1 expressed most in theca layer and granulosa layer with 2~4 mm, at the lowest in theca layer of F5 and granulosa layer of F1. [Conclusion] AKR1D1 may play an important role in the recruitment, selection, screening, atresia and ovulation of the follicle by regulating the dynamic balance of steroid hormones.

Key words: goose, AKR1D1 gene, coding domain sequence (CDS), real-time quantitative PCR