关键词: 玉米/大豆套作, 根系分隔, 地上部生物量, 固氮酶活性, 吸氮量

Abstract: [Objective] To determine the effects of different varieties and separation on nodule nitrogenase activity and nitrogen uptake in soybean in maize/soybean intercropping system, provide a reference to perfect the theory of nitrogen high-efficiency in intercropping systems. [Method] According to the root separation pot experiment, studied on the effect of three root barriers(without separation, separate by nylon net, separate by plastic membrane) on nitrogenase activity, aboveground biomass and nitrogen uptake in strong nodulation soybean varieties ‘NTS1007’ and weakness nodulation soybean varieties ‘southern bean 12’. [Results] The aboveground biomass, nitrogen uptake and nodule nitrogenase activity of ‘NTS1007’ were significant higher than ‘southern bean 12’, respectively, but the grain yield and nitrogen uptake were 48.9% and 55.3%lower than ‘southern bean 12’, respectively. Under without separate treatment, nodule nitrogenase activity, aboveground biomass and nitrogen uptake were significant higher than entirely separate. In R1, compare with entirely separate, nodule nitrogenase activity, aboveground biomass and nitrogen uptake were 26.11%, 43.4%, 18.5% higher in without separate treatment, and in R4, nodule nitrogenase activity, aboveground biomass and nitrogen uptake were 32.2%, 46%, 25.5% under without separate treatment.

Key words: maize/soybean intercropping, root separation, aboveground biomass, nitrogenase activity, nitrogen uptake