关键词: 山羊, 睾丸, 附睾, IL-1β, IL-6

Abstract: [Objective] To examine the location and distribution of Interleukin-1beta (IL-1β)and IL-6 in the testis, head and tail of epididymis of Jianchang black goat and the developmental expression levels of IL-1β and IL-6. [Method] The tissues of the testis and epididymis of 16 goats were collected from the 55-day, 104-day, 300-day and 1 140-day old (4repeats in each age group)to immunostain IL-1β and IL-6 respectively. [Results] The results showed that IL-1β and IL-6 were detected in spermatogonium of seminal tubes from 55-day, 104-day, 300-day and 1 140-day old goats, and IL-1β was localized in the connective tissue of testis from 55-day and 104-day old goats, and IL-6was expressed in the basal layer of seminal tube from 300-day and 1 140-day old goats. In the head and tail of epididymis, IL-1β and IL-6 were localized both in basal cells and ciliated columnar epithelium. The expression levels of IL-1β and IL-6 had a significant decrease from 55-day to 300-day in spermatogonium (P<0.05), while they had significant increases in develop-mental time (P<0.05)in connective tissue of the testis and basal cell of seminal tube. The change in expression level of IL-1β and IL-6in the head of epididymis was not significant in developmental time (P>0.05). However, IL-1β expression level at 300-day of ciliated columnar epithelium in the tail of epididymis was significantly lower than that at 55-day and 105-day (P<0.05), and expression level of IL-6in ciliated columnar epithelium of the tail of epididymis at 105-day was significantly higher than that at 55-day (P<0.05). [Conclusion] Both of IL-1β and IL-6were detected in the testis and epididymis of Jianchang black goat, and their expression levels were associated with developmental time.

Key words: Jianchang black goat, testis, epididymis, interleukin-1beta, interleukin 6