关键词: 雅安藏茶, 活性级分, 高通量筛选, 胃蛋白酶活性

Abstract: [Objective] The aim of this study was to explore the promoting effect of Ya'an Tibetan tea on pepsin. The results may provide a theoretical basis for physiological mechanism of Ya'an Tibetan tea. [Method] Seven kinds of Ya'an Tibetan tea fractions were separated by organic solvents, and water extract and major components were analyzed for the evaluation of the active ingredient to promote the pepsin by ELIASA and enzyme reaction system. [Results] There were significant dose-effect relationship between concentration of water extract (0.04~0.39mg/mL), major components (0.03~0.23mg/mL)from Ya'an Tibetan tea and acceleration of pepsin activity. Compared to the multiple activation of pepsin, the Activation ratio of four fractions (TFs, Cs, TRsⅡ, TRsI)from Ya'an Tibetan tea were 5.26, 4.40, 2.52 and 2.19, respectively. [Conclusion] Combined with principal component analysis, the main active ingredients to promote pepsin activity were TFs, catechin and TRs.

Key words: Ya'an Tibetan tea, active fraction, high-throughput screening, pepsin activity