关键词: 瓦氏黄颡鱼, 外周血, 生理指标, 显微观察

Abstract: [Objective] The research investigated blood physiological indexes, and constitute and microstructure of peripheral blood cell in Pelteobagrus vachelli. [Method] The blood physiological indexes were determined by count, cyanmethemoglobin determination and concentration gradient methods including red and white blood cells number, hemoglobin content and erythrocyte osmotic fragility. The differential leucocytes were counted, and the sizes of blood cells were measured through observing the peripheral blood cells smears which were stained by Wright's and Gimsa's stain. [Results] The results indicated that the number of red blood cells, white blood cells and hemoglobin content were 1.91×10¹²/L, 4.19×10⁹/L, and 60.89g/L. The total number of erythrocytes was significantly higher than white blood cells;hematocrit and erythrocyte sedimentation rate were 0.45L/L and 20.64mm/h;the minimum and maximum erythrocyte osmotic fragility were (0.45±0.01)% and (0.27±0.01)%, amplitude for resistance was (0.18±0.01)%. On the stained smears, five major cell types were recognized:erythrocytes, neutrophils, lymphocytes, monocytes, and thrombocytes;some immature blood cells and direct dividing of erythrocytes were also be observed, while eosinophils and basophils were not found;among these cells, the sizes of blood cell was:erythrocytes > monocytes > neutrophils > large lymphocytes > small lymphocytes > thrombocytes;differential leucocyte counts showed that the thrombocytes were the most abundant cell type after erythrocytes followed by small lymphocytes, neutrophils, large lymphocytes, and monocytes.

Key words: Pelteobagrus vachelli, peripheral blood, physiological indexes, microexamination