关键词: 鸭, YAP1基因, 生物信息学分析, 荧光定量PCR

Abstract: [Objective] The study was to clone the duck YAP1 gene cDNA coding domain sequence and predict its structure, function and expression profiles. [Method] We took Peking duck for material, using RT-PCR cloned the duck YAP1 coding domain sequence (CDS), predicted its protein structure and functions over several bioinformatics tools and analyzed its expression in 10 tissues of duck using real-time PCR. [Results] The results showed that the duck YAP1 CDS consists of 1 248 nucleotides that encode 415 amino acids residues, and the amino acid homology of duck YAP1 shares 99.7% identity with chicken. The analysis of amino acid sequence revealed that the duck YAP1 encoded water-soluble protein and its relative molecular weight was 45 417.5 Da. Subcellular location of YAP1 was in the nucleus, and it did not belong to the secreted protein. The YAP1 protein contained 30 phosphorylation sites, 30 glycosylation sites and 2 WW domains which are relatively conserved in species. The secondary structure of YAP1 was mainly composed of random coil, while the tertiary structure of domain area showed a forniciform helix structure. The result of real-time PCR revealed that duck YAP1 expresses most in pancreas. [Conclusion] According to the results and the analyses, the structure and the function of YAP1 in avian have a relatively high identity with which in mammals.

Key words: duck, YAP1 gene, bioinformatics analysis, real-time PCR