关键词: 戊糖片球菌, 培养基, 发酵条件, 优化

Abstract: [Objective] To harvest the fermentation product of Pediococcus pentosaceus C1C-4 with high efficiency and quantity. [Method] We performed series of single factor experiments and an orthogonal test to optimize the culture media, fermentation conditions, and product storage temperature by counting the number of living bacterium of Pediococcus pentosaceus C1C-4. [Results] The number of living bacterium was significantly influenced by nitrogen source and carbon source (P<0.05). The ingredients of optimal culture medium were obtained as soybean powder 35g/L, sucrose 35g/L, and mineral salt 1.5g/L, with a highest figure of 6.02×10⁹ CFU/mL. The growth curve was tested by using the optimized culture media. The results indicated that Pediococcus pentosaceus grows slowly at early stage (0-8h), turns into exponential growth in 12-24 h, and maintains considerable number in next 24 h, with highest number of living bacterium in 28h。We tested the effect of storage temperature on the number of living bacterium of bacterium powder. Compared with bacterium powder storing in 4℃, the number of living bacterium decreased more rapidly in 25℃ and 37℃, which suggested the bacterium powder should be stored in low temperature environment. [Conclusion] The optimization of culture media, fermentation conditions, and storage temperature provides a more effect way for pediococcus pentosaceus fermentation and storage, respectively.

Key words: Pediococcus pentosaceus, culture media, fermentation conditions, optimization