关键词: 节节麦, 高分子量谷蛋白基因, 克隆, 测序

Abstract: To understand the molecular bases for HMW glutenin subunit Dx5^t+Dy12^t endowing wheat flours with prominent end use qualities, the electrophoresis mobility of Dy12^t in this subunit combinations was determined by SDS-PAGE analysis and the gene for this subunit was isolated and sequenced. Results showed that the electrophoresis mobility of HMW glutenin Dy12^t of Aegilops tauschii and Dy12 of wheat are the same, while both are distinctively different from Dy10 of wheat. However, at amino acid sequence level, HMW glutenin Dy12^t differs from Dy10 only by four amino acid replacement, while they showed great difference with Dy12 by twelve amino acid replacements and two hexpeptide insertions and four amino acid insertions/deletions. The results suggested that the main reason for Ae. tauschii HMW glutenin Dx5^t+Dy12^t endowing wheat with good baking quality could ascribe to the high similarity between HMW glutenin Dy12^t and Dy10 at amino acid sequence level, which makes the quality potential of HMW glutenin subunit pair Dx5^t+Dy12^t is very like that of Dx5+Dy10.

Key words: Aegilops tauschii, HMW glutenin gene, cloning, sequencing