关键词: 微生物絮凝剂, 筛选, 培养条件

Abstract: A high efficient bioflocculant-producing strain HY1141 was screened from the 48 azotobacter bacterial strains. After determination of the optimal carbon and nitrogen source, its optimal medium and culture condition were screened by an orthogonal test. The results showed that the optimal carbon source is sucrose and the optimal nitrogen source is N₂. In the absence of exotic nitrogen, the optimal medium consists of sucrose 5 g/L, KH₂PO₄ 1.5 g/L and MgSO₄ 0.6 g/L. The optimal culture condition includes rotation speed 150 r/min, culture temperature 35℃, original medium pH 7.0, culture time 36 h. Under such conditions, the highest flocculating rate of flocculants produced by HY1141 to Gaoling clay suspension is as high as 96.4%.

Key words: microbial flocculant, screening, culture conditions