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四川农业大学学报 ›› 2007, Vol. 25 ›› Issue (03): 257-261.doi: 10.16036/j.issn.1000-2650.2007.03.001

• 研究论文 • 上一篇    下一篇

高效无机解磷细菌YM3-2S的分离和鉴定

赵珂, 陈强, 陈晓琴, 赵芯, 吴翔   

  1. 四川农业大学 资源环境学院, 四川 雅安 625014
  • 收稿日期:2007-03-02 出版日期:2007-09-30 发布日期:2017-03-04
  • 基金资助:
    四川农业大学科技创新基金(14304);四川省教育厅重点项目(2004A004)。

Isolation and Identification of a High Efficiency Phosphate-solubilizing Bacterium YM3-2S

ZHAO Ke, CHEN Qiang, CHEN Xiao-qin, ZHAO Xin, WU Xiang   

  1. College of Resource and Environment, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2007-03-02 Online:2007-09-30 Published:2017-03-04

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摘要： 从四川省不同地区的土壤样品进行了解磷细菌的分离和筛选,根据溶磷圈大小,获得了7株解磷细菌,并最终筛选到一株具有高效解磷能力的菌株YM3-2S,对磷矿粉的最大解磷量达40.96 mg/L,解磷率可达20%。根据其形态特征,生理生化特性和16S rDNA序列,将菌株YM3-2S鉴定为洋葱伯克氏菌(Burkholderia cepacia)。

关键词: 解磷细菌, 筛选, 生理生化特性, 16SrDNA序列

Abstract: Seven phosphorus-solubilizing bacterial strains were isolated from 30 soil samples collected from 7 different sites in Sichuan province, and a high efficiency phosphate-solublizing bacterial strain named YM3-2S was obtained. The hig hest phosphate-releasing amount of strain YM3-2S was 40.96 mg/L, and the phosphate-releasing ratio was 20%. Based on the results of morphology, physiological and biochemical properties and phylogenetic analysis of 16S rDNA sequence, strain YM3-2S was identified as Burkholderia cepacia.

Key words: phosphate-solubilizing bacteria, screening, physiological and biochemical properties, 16S rDNA sequence

中图分类号: 

  • Q93-331
ISSN 1000-2650
CN 51-1281/S
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