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四川农业大学学报 ›› 2007, Vol. 25 ›› Issue (02): 117-120.doi: 10.16036/j.issn.1000-2650.2007.02.002

• 研究论文 •    下一篇

水蔓菁组织培养及植株再生研究

刘帆1, 汤福义2, 田孟良1, 乔晓1, 官宇1   

  1. 1. 四川农业大学农学院, 四川 雅安 625014;
    2. 四川农业大学林学园艺学院, 四川 雅安 625014
  • 收稿日期:2007-03-30 出版日期:2007-06-30 发布日期:2017-03-03
  • 基金资助:
    四川农业大学青年科技创新基金。

Tissue Culture and Plantlet Regeneration of Veronica linariifolia subsp. Dilatata

LIU Fan1, TANG Fu-yi2, TIAN Meng-liang1, QIAO Xiao1, GUAN Yu1   

  1. 1. College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2007-03-30 Online:2007-06-30 Published:2017-03-03

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摘要： 采用组织培养方法,设置不同激素配比的培养基对水蔓菁(Veronica linariifolia subsp. dilatata)不同外植体叶片、茎尖及茎段进了行研究。结果表明MS+6-BA 2.0 mg/L+NAA 0.5 mg/L诱导愈伤组织效果最佳,其中以茎尖的诱导效果优于其他外植体;MS+6-BA 2.0 mg/L+NAA 0.05 mg/L诱导愈伤组织丛生芽的分化效果最佳;1/2 MS+IBA 0.5 mg/L诱导丛生芽的生根效果最佳。

关键词: 水蔓菁, 组织培养, 植株再生

Abstract: To establish a high efficiency plant regeneration system of Veronica linariifolia., callus, rosette buds, and roots were induced by different combinations of hormone from leaves, stem-tips and stems of Veronica linariifolia.The results showed that the medium of MS+6-BA 2.0 mg/L+NAA 0.5 mg/L was the best for callus induction, the medium of MS+6-BA 2.0 mg/L+NAA 0.05 mg/L was the best for bud differentiation, and the medium of 1/2 MS+IBA 0.5 mg/L was the beste for rooting.

Key words: Veronica linariifolia, tissue culture, plant regeneration

中图分类号: 

  • S682.3
ISSN 1000-2650
CN 51-1281/S
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