关键词: 栗疫菌, 板栗, 毒素

Abstract: Chestnut blight caused by Endothia parasitica, is one of forest plant quarantine pests. Toxin is one of important pathogenic factors to plants. Shake culture and still method were applied in this experiment and the substrate was PD. Two bioassay methods were conducted in this experiment, namely dipping method and needle puncture. The experiment show that twigs and leaves indicate distinct symptom. So Endothia parasitica can produce bioactive phytotoxin, PD medium filtrate is suitable for isolate growth and toxin production, shake culture is better than still method;the second day with dipping method twigs and leaves indicates pathological changes and wilt, the first day with needle puncture plant indicates symptom. So needle puncture is better than dipping method and leaves are more sensitive than twigs.

Key words: Endothia parasitica, chestnut, toxin