四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (04): 402-404.doi: 10.16036/j.issn.1000-2650.2006.04.007

• 研究论文 • 上一篇    下一篇

脐橙果实发育过程中有机酸代谢相关酶的研究

龚荣高1, 吕秀兰1, 张光伦1, 胡世学2   

  1. 1. 四川农业大学 林学园艺学院, 四川 雅安 625014;
    2. 重庆市奉节县农业局, 重庆 奉节 404600
  • 收稿日期:2006-09-12 出版日期:2006-12-31 发布日期:2017-03-06
  • 基金资助:
    四川省农业厅重点支持项目(2002-08-03-02);四川省教育厅青年基金(2006B008)

Study on the Organic Acid-metabolizing Enzymes in Robertson Navel Orange during the Fruit Development

GONG Rong-gao1, LÜ Xiu-lan1, ZHANG Guang-lun1, HU Shi-xue2   

  1. 1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Agricultural Bureau of Fengjie County, Chongqing 404606, China
  • Received:2006-09-12 Online:2006-12-31 Published:2017-03-06

摘要: 以罗伯逊脐橙为试材,研究了脐橙果实有机酸代谢相关酶的活性变化动态,及其与果实有机酸积累关系,旨在探索影响脐橙果实有机酸含量的重要酶。结果表明:果实有机酸的积累期,CS、PEPC活性随有机酸积累而增大,并呈显著正相关;有机酸降解期,IDH的活性与有机酸含量呈极显著负相关;而MDH和ACO对酸含量的影响小。因而可认为PEPC和CS是影响果实有机酸合成的重要酶,而影响有机酸分解的重要酶则是IDH。

关键词: 脐橙, 有机酸积累, 代谢相关酶

Abstract: ‘Robertson Navel’ orange(Citrus sinesis Osb.) fruits were used to study organic acid-metabolizing enzymes during the fruit development, especially the changes in activities of citrate synthase(CS), phosphoenolpyruvatecarboxylase(PEPC), cis-aconitase(ACO), isocitrate dehydrogenase(IDH) and malate dehydrogenase(MDH), in order to find out the key enzymes which influences the organic acid content in navel fruit. The results show that CS and PEPC play an important role in organic acid accumulation during the early stage of the fruit development, while IDH is the key enzyme that cleave citrate from 120 days after full bloom to the fruit ripening. However, MDH and CAO are not the key enzymes that influence the organic acid accumulation.

Key words: navel orange, organic acid accumulation, metabolizing enzyme

中图分类号: 

  • S666