关键词: 筇竹, 遗传多样性, 优化, CTAB, RAPD

Abstract: The Qiongzhuea tumidinoda RAPD reaction system optimized with yibin is established in ordered to be used in Qiongzhuea tumidinoda hereditary variety analysis. The genomic DNA of Qiongzhuea tumidinoda is extracted with improved CTAB method. The effect of content of Mg²⁺, dNTP, DNA templates, primers and DNA polymerase on experimental results is tested and the optimal reaction system of RAPD Qiongzhuea tumidinoda is determined as the following: 10×Taq polymerase corresponding buffer(2 μL), 1.25 U Taq polymerase, 75 ng template DNA, 45 ng primer, 1.88 mmol/L MgCl₂, 0.19 mmol/L dNTP in total 20 μL reaction volume. This is to use RAPD marker to settle solid foundation for the classification of Qiongzhuea tumidinoda again on the molecular level.

Key words: Qiongzhuea tumidinoda, hereditary variety analysis, optimization, CTAB, RAPD