四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (02): 218-221.doi: 10.16036/j.issn.1000-2650.2006.02.021

• 研究论文 • 上一篇    下一篇

4个山羊品种FSHβ基因部分序列的比较分析

李利, 张红平, 吴登俊   

  1. 四川农业大学 动物科技学院, 四川 雅安 625014
  • 收稿日期:2005-12-05 出版日期:2006-06-30 发布日期:2017-03-06
  • 基金资助:
    国家863基金项目(编号:2002AA242051)

Comparative Study on Partial Sequence of Follicle Stimulating Hormone Beta Subunit Gene from Four Goat Breeds

LI Li, ZHANG Hong-ping, WU Deng-jun   

  1. College of Animal and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2005-12-05 Online:2006-06-30 Published:2017-03-06

摘要: 利用牛、绵羊等动物FSHβ亚基基因序列设计引物,采用PCR法扩增并测定了马头山羊(2只)、昭通山羊(2只)、榕江小香羊(1只)以及雷州山羊(3只)4个品种FSHβ亚基基因部分序列(GenBank登录号:AY853276)。在测定出的FSHβ亚基基因732 bp序列中,包括第一外显子全序列(1~61 bp),第一内含子全序列(62~703 bp)以及第二外显子部分序列(704~732 bp),并且序列中A+T含量(66.12%)高于G+C(33.88%)。测定出的第二外显子部分编码序列(21 bp)共编码7个氨基酸。序列比对发现,除了一只昭通山羊的第78位为碱基A以外,其余个体的此位点碱基缺失,此位点与产羔率之间的关系需做进一步的研究。

关键词: 山羊, FSHβ基因, 序列分析, 产羔率

Abstract: According to the FSHβ gene sequences of bovine and sheep, the primers are designed and the partial sequences of FSHβ gene from 2 Matou goats, 2 Zhaotong goats, 1 Rongjiang-xiao-xiang goat and 3 Leizhou goats are studied(Accession No.AY853276). It includes the complete first exon(1~61 bp), first intron(62~703 bp) and partial sequence of exon 2(704~732 bp) in the 732 base pairs of FSHβ gene sequence, and the content of A+T(66.12%) is higher than G+C(33.88%). The coding sequence of exon 2 codes 7 amino acids. Only one sequence from a Zhaotong goat has A at 78th loci, others lack this nucleotide, the relationship between the mutation loci and the birth rate is to be studied deeply in the future.

Key words: goat, FSHβ gene, sequence analysis, lambing percentage

中图分类号: 

  • S827.9