关键词: 黑麦, 普通小麦, γ-醇溶蛋白, γ-黑麦碱, 分子标记

Abstract: A pair of primers are designed according to the DNA sequences of 75K γ-secalin of rye. After PCR amplification with the primers, one sharp band with a length of about 400 bp is respectively produced in all eight different common wheat lines. Two of them are respectively sequenced and submitted to GenBank(accession numbers DQ432029 and DQ432030). DNA sequences of DQ432029 and DQ432030 are identical, which show strong homology to the partial coding sequences(CDS) of γ-gliadin. However, the significant differences of DQ432029 from known sequences suggest that DQ432029 represents a fragment of a novel type γ-gliadin from wheat. Due to the specific amplification in all materials used in this study, the corresponding PCR production can be used as a marker for the type of gliadin gene.

Key words: rye, common wheat, γ-gliadin, γ-secalin, molecular marker