四川农业大学学报 ›› 2005, Vol. 23 ›› Issue (03): 258-263.doi: 10.16036/j.issn.1000-2650.2005.03.002

• 研究论文 • 上一篇    下一篇

波兰小麦(Triticum polonicum L.)高分子麦谷蛋白亚基多样性分析

刘虹, 张志清, 颜泽洪, 魏育明, 郑有良   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2005-05-01 出版日期:2005-09-30 发布日期:2017-04-21
  • 基金资助:
    高等学校全国优秀博士学位论文作者专项资金(基金编号:200357,200458)。

Allelic Variations of High Molecular Weight Glutenin Subunits in Triticum polonicum L.

LIU Hong, ZHANG Zhi-qing, YAN Ze-hong, WEI Yu-ming, ZHENG You-liang   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2005-05-01 Online:2005-09-30 Published:2017-04-21

摘要: 利用SDS-PAGE电泳技术鉴定和分析了72份不同来源的波兰小麦高分子量谷蛋白亚基组成。结果表明,供试波兰小麦材料中有10种亚基组合方式。其中,亚基组合类型(N,20)出现的频率最高,高达41.67%;其次是(Null,7)亚基组合。在各位点的等位变异中,Glu-A1位点出现3种等位变异,以Null出现的频率最高(84.7%);而在Glu-B1位点上检测到了5种等位变异。不同地理来源的波兰小麦高分子量谷蛋白亚基组合类型从1到10种不等,尤其是来源于欧洲地区的波兰小麦,具有检测到的所有亚基组合类型,存在较高多样性。

关键词: 波兰小麦, 高分子量谷蛋白亚基, SDS-PAGE, 多样性

Abstract: Allelic variation of high molecular weight glutenin subunits (HMW-GS) among 72 accessions of Triticum polonicum L.were analyzed by using one dimensional SDS-PAGE. A total of 10 subunit combinations were observed. Among which, the highest frequency of subunit combinations (N, 20) was 41.67%, following the second frequent was (N, 7) subunit combination. Three and 5 allelic variations were found in Glu-A1 and Glu-B1 locus, respectively. The most abundant subunit combinations were detected in those materials from Europe.

Key words: Triticum polonicum L., high molecular weight glutenin subunits (HMW-GS), SDS-PAGE, genetic diversity

中图分类号: 

  • S512.1