关键词: 长寿花, 带芽茎段, 离体繁殖

Abstract: Stems with buds of Kalanchoe blossfeldiana were used as explants. The establishment of explants, proliferation and rooting of shoots and transplantation of plantlets were studied. The results show that the optimum initiation medium is MS+6-BA 0.5 mg/L+NAA 0.1 mg/L, with 100.0% initiation rate obtained. Shoots multiply more effectively on the media of combinations of 6-BA, NAA and GA₃. The shoots cultured on the medium of MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+GA₃ 0.1 mg/L result in 9.3 times of proliferation. The semisolid medium of MS with 6-BA 2.0 mg/L, GA₃ 0.1 mg/L and AC 2.0 g/L is more effective than solid medium for the multiplication and rooting for shoots, each explant produces up 5.5 seedlings and all the explants root successfully. The plantlets are transplanted into nutrient soil with 100.0% survival ratio.

Key words: Kalanchoe blossfeldiana, stem with buds, propagation in vitro