关键词: 稻瘟病菌, 交配型, 育性, PCR检测, 四川

Abstract: In this research, standard isolates of mating types (MAT-1 and MAT-2) in Magnaporthe grisea provided by IRRI (2539w and 6023) and IRR (KA3 and Guy 11) are paired with 141 isolates from different varieties and districts in Sichuan province, China, to detect the presence of both mating types in the area. PCR amplification with the special primers 5'-TCAGCTCGCCCAAATCAACAAT-3', 5'-ACTCAA GACCCGGCACGAACAT-3'(MAT-1), 5'-GAGTTGCCTGCCCGCTTCTG-3' and 5'-GGCTTG GTCGTTGGGGATTGT-3'(MAT-2) based on the basal sequence of gene MAT-1 and MAT-2 is also conducted for the verification of such presence and the validity of PCR detection of mating types in M.grisea. 36.17% of the tested isolates are fertile with respective 27 and 24 isolates belonging to MAT1-1 and MAT1-2 while 32 isolates are found to be hermaphroditic. Isolates of different mating types are found at different sampling sites or even from same paddy fields. Differences in the fertility of standard isolates used in the experiments are found to be responsible for the differences from the previous reports about the low frequency of MAT-2 compared to that of MAT-1 in Sichuan population of M.grisea. The mating types of 75% of isolates detected by PCR amplification are the same as that obtained by pairing with standard isolates. The combination of PCR detection and cross with standard isolates is suggested to be used for the mating type detection.

Key words: Magnaporthe grisea, mating type, fertility, PCR detection, Sichuan