四川农业大学学报 ›› 2005, Vol. 23 ›› Issue (01): 15-18.doi: 10.16036/j.issn.1000-2650.2005.01.004

• 研究论文 • 上一篇    下一篇

小麦与八倍体小偃麦杂种结合辐射诱导后代的细胞学分析

刘晓东, 傅体华, 任正隆   

  1. 四川农业大学 农学院, 四川 雅安 625014
  • 收稿日期:2005-02-25 出版日期:2005-03-31 发布日期:2017-04-21
  • 基金资助:
    国家自然科学基金项目(30370770);四川省学术技术带头人基金项目。

Cytology of the Progenies Derived from Combination of Hybrid between Wheat and Octoploid Agroticum with Radiation

LIU Xiao-dong, FU Ti-hua, REN Zheng-long   

  1. College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2005-02-25 Online:2005-03-31 Published:2017-04-21

摘要: 为了转移偃麦草属的有利基因,利用西南推广的小麦品种川麦107为母本,来源于中间偃麦草和长穗偃麦的两个八倍体小偃麦杂交所得的F1为父本。对其杂种F2代种子进行辐射处理,然后开放授粉自交多代,大群体混合选择。在F2M6世代,随机选择148个单株进行花粉母细胞减数分裂观察和分析。结果表明:在该世代染色体数目变化为41~46条,有相当一部分材料在细胞学上基本稳定,它们的减数分裂细胞中期Ⅰ中绝大多数细胞的染色体以二价体形式存在,减数分裂正常。但也有一些材料在减数分裂中期Ⅰ出现较高频率的三价体,四价体,甚至多价体,并伴随有较多的单价体,某些细胞中存在减数分裂异常现象,如染色体桥,易位环,落后染色体等,表明极有可能从这些材料中筛选出易位系。另外还分离出十多个可能的小麦-偃麦草附加系。

关键词: 普通小麦, 偃麦草, 减数分裂, 外源易位系, 外源附加系

Abstract: The hybrid F1 from the cross between wheat-Ag.intermedium and wheat-Ag.elongata was used as male, to transfer desirable genes of Agropyrum into the wheat variety, Chuanmai 107, recently released in southwest China. The seeds of F2 generation were γ-radiation treated with 4 000 roentgen. The chromosomal behavior at meiosis in pollen mother cells of 148 plants from F2M6 progeny was observed. The results indicate that the chromosome number of observed plants ranges from 2n=41 to 2n=46, of which the plants of 2n=42 are more frequent. Most of plants with 2n=42 have stable cytology characteristics, however, trivalents, and other multivalents are also recorded at MI in other plants with 2n=42, suggesting the translocations with desirable genes might be selected from these plant progenies. In addition, more than 10 wheat-Agropyrum alien addition lines have been obtained by preliminarily cytological identification.

Key words: Triticum aestivum L., Agropyrum, meiosis, alien translocation line, alien addition line

中图分类号: 

  • S512.1