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四川农业大学学报 ›› 2004, Vol. 22 ›› Issue (03): 252-256.doi: 10.16036/j.issn.1000-2650.2004.03.013

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乙脑病毒CN株PrM/E基因的克隆及序列分析

汤德元1,2, 郭万柱1, 徐志文1, 颜其贵1, 王印1, 王晓玉1   

  1. 1. 四川农业大学 动物科技学院, 四川 雅安 625014;
    2. 贵州大学 动物科学学院, 贵州 贵阳 550025
  • 收稿日期:2004-05-20 出版日期:2004-09-30 发布日期:2017-03-04

Cloning and Sequencing of PrM/E Genes of Japanese Encephalitis Virus(JEV) CN Strain

TANG De-yuan1,2, GUO Wan-zhu1, XU Zhi-wen1, Yan Qi-gui1, Wang Yin1, Wang Xiao-yu1   

  1. 1. College of Animal and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China
  • Received:2004-05-20 Online:2004-09-30 Published:2017-03-04

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摘要： 通过RT-PCR扩增乙型脑炎病毒CN株主要抗原基因PrM/E,并将PrM/E基因克隆、测序后,与GenbanK发表的JEVSA14(U14163)基因序列进行比较分析,发现CN株与JEVSA14强毒株的同源性为98%。在JEV基因组5'端477~2477的长2000nt的决定JEV抗原性的PrM/E区中,CN株与SA14株有40个碱基不同,其中28个碱基突变为同义突变,另外12个为错义突变,导致相应的氨基酸序列(666个)中的4个氨基酸发生了突变,其中有2个氨基酸出现在含有关键的抗原决定簇的E蛋白内,但不影响其功能表达

关键词: 日本乙型脑炎病毒, PrM/E基因克隆, 核酸序列

Abstract: This paper reports the cloning of the PrM/E gene of the Japanese Enceplialitis Virus(JEV) CN strain by RT-PCR and analysis of the gene sequence. Comparison of the gene sequence with that of the JEV SA14 strain demonstrates 98% sequence homology. In the 2 Kb genome region of the virus, between nucleotides 477~2477, 40 base pairs differe between the two strain. Only 12 of these differences are missense mutations that result in 4 amino acid differences in the protein sequences. Two of the amino acid substitutions occur in the region encoding for the E protein and do not cause a change in the activity of this protein between the two strains of JEV.

Key words: Japanese encephalitis virus, PrM/E genes cloning, sequence analysis

中图分类号: 

  • R373.31
ISSN 1000-2650
CN 51-1281/S
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