关键词: 石灰花楸, 组织培养

Abstract: By using stems with buds of Sorbus folgneri(Schneid.) Rehd as materials, the experiment was made to find out the best culture process and the proper culture medium. The technical system of tissue culture for rapid propagation was established. The results are as follows. The optimal initiation medium is MS+2.0 mg/L 6-BA+0.5 mg/L NAA, and when the stem is isolated in spring, the rate of initiation is over 90%. The optimal subculture medium is 1/2 MS+1.5 mg/L 6-BA+1.0 mg/L NAA, the multiplication rate of shoot clumps can surpass 4.00. The proper rooting medium is 1/2 MS+1.0 mg/L IBA+0.1 mg/L NAA+0.5 mg/L Ac+10 g/L sugar and the rooting rate can be up to 75%.

Key words: Sorbus folgneri(Schneid.) Rehd, tissue culture