关键词: 小麦, 成熟胚, 植株再生, 植物激素

Abstract: Mature wheat embryos of new wheat varieties Chuannong 17 and 12 were cultured in vitro. The results show that 2 mg/L 2, 4-D is beneficial to the formation of totipotent calli. 5 mg/L KT is beneficial to plant regeneration(The regeneration frequencies of the two cultivars are 73.63%, 89.74% respectively). High concentration of 2, 4-D(4~8 mg/L) has no bad effect on callus formation, but they inhibit plant regeneration. This kind of inhibition is resisted by KT, but in the initiation media supplemented with 2 mg/L 2, 4-D this kind of resistance disappears.

Key words: wheat, mature embryo, plant regeneration, phytohormone