关键词: 西藏小麦, 地方品种, 醇溶蛋白, 等位基因, A-PAGE

Abstract: Tibet wheat landraces were investigated for their gliadin allele compositions by acidic polyacrylamide gel electrophoresis(A-PAGE). The results suggest that there is high variance among six major loci of gliadin in Tibet wheat. There are 98 types of alleles and 114 gliadin combinations. The average Nei's genetic variation index(H) of six main loci is up to 0.854. The genetic variation indexes of the first and the sixth group chromosomes have the similar trend. A's is the highest, B's is the secondary and D's is the lowest. The allele types in six main gliadin loci vary from 7 to 24, with average of 16.33. The highest frequency at loci in Gli-A1, Gli-B1, Gli-D1, Gli-A2, Gli-B2 and Gli-D2 is urt3(14.62%), k(15.38%), a(40.00%), o(14.62%), a(22.38%) and a(40.00%), respectively. There are 4, 2 and 2 kinds of gliadin blocks in each of the Gli-A1, Gli-B1 and Gli-A2 loci have not been characterized. At the Gli-B1 loci, three Tibet wheat landrace can not encode any expressed gliadin band.

Key words: Tibet wheat, landrace, gliadin, allele, A-PAGE