关键词: 金花梨, DNA提取, RAPD分析

Abstract: The CTAB method was definitely improved by different doses of Vc appended when the sample of tender bud of Jinhua pear was triturated. And then the isolation effect of genomic DNA from mature leaf, shoot and tender bud of Jinhua pear was studied respectively by improved CTAB method. After comparing the quantity and quality of genomic DNA extracted from different materials, the high-quality genome DNA of Jinhua pear and its 18 varieties was used as templates and amplified with 40 random primers by using RAPD. The result shows that with the dose of 0.10 g/g of Vc added when the sample is triturated, the high-quality of genomic DNA can be extracted from tender bud, and the genomic DNA can be extracted from mature leaf, shoot and tender bud all can be used for RAPD analysis. Fragments are amplified on different templates with most of 40 random primers. Only five primers get better result on all templates. According to the results of RAPD, the genetic relationship among Jinhua pear and its 18 varieties is examined for classification of Jinhua pear.

Key words: Jinhua pear, DNA extraction, RAPD analysis