关键词: 组织培养, 小麦, 育种

Abstract: The wheat F₅ lines originated from different combinations were analyzed for determining their tissue culture capacity. The combination 1 contains two parents with excellent ability in tissue culture, but combination 2 does not. More significant lines, which exhibited excellent characters in tissue culture, such as the days of calluing at 50%, the rate of total calli induction, the rate of root formation, the rate of shoot information, were observed in combination 1 than combination 2. The results indicate that the F₅ lines contain the genes of tissue culture characters from their wheat parents, in despite of selection lack in F₂~F₄ for tissue culture characters. In wheat breeding for transgenic receptors it is an approach to select tissue culture abilities with excellent agronomic characteristics in F₅ plant lines.

Key words: tissue culture, wheat, plant breeding